Počet záznamů: 1
Bordetella pertussis Adenylate Cyclase Toxin Disrupts Functional Integrity of Bronchial Epithelial Layers
- 1.
SYSNO ASEP 0488787 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bordetella pertussis Adenylate Cyclase Toxin Disrupts Functional Integrity of Bronchial Epithelial Layers Tvůrce(i) Hasan, Shakir (MBU-M)
Kulkarni, N.N. (IS)
Asbjarnarson, A. (IS)
Linhartová, Irena (MBU-M) RID, ORCID
Osička, Radim (MBU-M) RID, ORCID
Šebo, Peter (MBU-M) RID, ORCID
Gudmundsson, H. (IS)Číslo článku e00445-17 Zdroj.dok. Infection and Immunity. - : American Society for Microbiology - ISSN 0019-9567
Roč. 86, č. 3 (2018)Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Bordetella pertussis ; airway epithelia ; CyaA Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA15-09157S GA ČR - Grantová agentura ČR NV16-28126A GA MZd - Ministerstvo zdravotnictví LM2015064 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA18-20621S GA ČR - Grantová agentura ČR EF16_013/0001818 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000425510700008 EID SCOPUS 85042315699 DOI 10.1128/IAI.00445-17 Anotace The airway epithelium restricts the penetration of inhaled pathogens into the underlying tissue and plays a crucial role in the innate immune defense against respiratory infections. The whooping cough agent, Bordetella pertussis, adheres to ciliated cells of the human airway epithelium and subverts its defense functions through the action of secreted toxins and other virulence factors. We examined the impact of B. pertussis infection and of adenylate cyclase toxin-hemolysin (CyaA) action on the functional integrity of human bronchial epithelial cells cultured at the air-liquid interface (ALI). B. pertussis adhesion to the apical surface of polarized pseudostratified VA10 cell layers provoked a disruption of tight junctions and caused a drop in transepithelial electrical resistance (TEER). The reduction of TEER depended on the capacity of the secreted CyaA toxin to elicit cAMP signaling in epithelial cells through its adenylyl cyclase enzyme activity. Both purified CyaA and cAMP-signaling drugs triggered a decrease in the TEER of VA10 cell layers. Toxin-produced cAMP signaling caused actin cytoskeleton rearrangement and induced mucin 5AC production and interleukin-6 (IL-6) secretion, while it inhibited the IL-17A-induced secretion of the IL-8 chemokine and of the antimicrobial peptide beta-defensin 2. These results indicate that CyaA toxin activity compromises the barrier and innate immune functions of Bordetella-infected airway epithelia. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
Počet záznamů: 1