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Exocyst subunit EXO70H4 has a specific role in callose synthase secretion and silica accumulation

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    SYSNO ASEP0488738
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevExocyst subunit EXO70H4 has a specific role in callose synthase secretion and silica accumulation
    Tvůrce(i) Kulich, I. (CZ)
    Vojtíková, Z. (CZ)
    Sabol, P. (CZ)
    Ortmannová, Jitka (UEB-Q) ORCID
    Neděla, Vilém (UPT-D) RID, ORCID, SAI
    Tihlaříková, Eva (UPT-D) RID, ORCID, SAI
    Žárský, Viktor (UEB-Q) RID, ORCID
    Celkový počet autorů7
    Zdroj.dok.Plant Physiology - ISSN 0032-0889
    Roč. 176, č. 3 (2018), s. 2040-2051
    Poč.str.12 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaCOMPLETE PENETRATION RESISTANCE ; SCANNING-ELECTRON-MICROSCOPE ; POWDERY MILDEW
    Vědní obor RIVEB - Genetika a molekulární biologie
    Obor OECDCell biology
    Vědní obor RIV – spolupráceÚstav přístrojové techniky - Elektronika a optoelektronika, elektrotechnika
    Institucionální podporaUEB-Q - RVO:61389030 ; UPT-D - RVO:68081731
    UT WOS000426848300018
    EID SCOPUS85044003089
    DOI10.1104/pp.17.01693
    AnotaceBiogenesis of the plant secondary cell wall involves many important aspects, such as phenolic compound deposition and often silica encrustation. Previously, we demonstrated the importance of the exocyst subunit EXO70H4 for biogenesis of the trichome secondary cell wall, namely for deposition of the autofluorescent and callose-rich cell wall layer. Here, we reveal that EXO70H4-driven cell wall biogenesis is cons titutively active in the mature trichome, but also can be activated elsewhere upon pathogen attack, giving this study a broader significance with an overlap into phytopathology. To address the specificity of EXO70H4 among the EXO70 family, we complemented the exo70H4-1 mutant by 18 different Arabidopsis (Arabidopsis thaliana) EXO70 paralogs subcloned under the EXO70H4 promoter. Only EXO70H4 had the capacity to rescue the exo70H4-1 trichome phenotype. Callose deposition phenotype of exo70H4-1 mutant is caused by impaired secretion of PMR4, a callose synthase responsible for the synthesis of callose in the trichome. PMR4 colocalizes with EXO70H4 on plasma membrane microdomains that do not develop in the exo70H4-1 mutant. Using energy-dispersive x-ray microanalysis, we show that both EXO70H4- and PMR4-dependent callose deposition in the trichome are essential for cell wall silicification.
    PracovištěÚstav experimentální botaniky
    KontaktDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Rok sběru2019
Počet záznamů: 1