Počet záznamů: 1

EFA6 regulates selective polarised transport and axon regeneration from the axon initial segment

SYSNO ASEP	0487517
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	EFA6 regulates selective polarised transport and axon regeneration from the axon initial segment
Tvůrce(i)	Eva, R. (GB) Koseki, H. (GB) Kanamarlapudi, V. (GB) Fawcett, James (UEM-P)_ORCID
Zdroj.dok.	Journal of Cell Science. - : Company of Biologists - ISSN 0021-9533 Roč. 130, č. 21 (2017), s. 3663-3675
Poč.str.	13 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	axon regeneration ; axon transport ; neuronal polarisation
Vědní obor RIV	FH - Neurologie, neurochirurgie, neurovědy
Obor OECD	Neurosciences (including psychophysiology
Institucionální podpora	UEM-P - RVO:68378041
UT WOS	000414149400009
EID SCOPUS	85032824407
DOI	10.1242/jcs.207423
Anotace	Central nervous system (CNS) axons lose their intrinsic ability to regenerate upon maturity, whereas peripheral nervous system (PNS) axons do not. A key difference between these neuronal types is their ability to transport integrins into axons. Integrins can mediate PNS regeneration, but are excluded from adult CNS axons along with their Rab11 carriers. We reasoned that exclusion of the contents of Rab11 vesicles including integrins might contribute to the intrinsic inability of CNS neurons to regenerate, and investigated this by performing laser axotomy. We identify a novel regulator of selective axon transport and regeneration, the ARF6 guanine-nucleotide-exchange factor (GEF) EFA6 (also known as PSD). EFA6 exerts its effects from a location within the axon initial segment (AIS). EFA6 does not localise at the AIS in dorsal root ganglion (DRG) axons, and in these neurons, ARF6 activation is counteracted by an ARF GTPase-activating protein (GAP), which is absent from the CNS, ACAP1. Depleting EFA6 from cortical neurons permits endosomal integrin transport and enhances regeneration, whereas overexpressing EFA6 prevents DRG regeneration. Our results demonstrate that ARF6 is an intrinsic regulator of regenerative capacity, implicating EFA6 as a focal molecule linking the AIS, signalling and transport.
Pracoviště	Ústav experimentální medicíny
Kontakt	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Rok sběru	2018

Počet záznamů: 1