Počet záznamů: 1  

Analysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery

  1. 1.
    SYSNO ASEP0487351
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevAnalysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery
    Tvůrce(i) Živicová, V. (CZ)
    Lacina, L. (CZ)
    Mateu, R. (CZ)
    Smetana, K. (CZ)
    Kavkova, R. (CZ)
    Krejci, E.D. (CZ)
    Grim, M. (CZ)
    Kvasilová, A. (CZ)
    Borský, J. (CZ)
    Strnad, Hynek (UMG-J) RID
    Hradilová, Miluše (UMG-J)
    Šáchová, Jana (UMG-J)
    Kolář, Michal (UMG-J) RID
    Dvořánková, B. (CZ)
    Celkový počet autorů14
    Zdroj.dok.International Journal of Molecular Medicine. - : Spandidos Publications - ISSN 1107-3756
    Roč. 40, č. 5 (2017), s. 1323-1334
    Poč.str.12 s.
    Jazyk dok.eng - angličtina
    Země vyd.GR - Řecko
    Klíč. slovadermal fibroblasts ; myofibroblast ; neonatal healing ; trandforming growth factor-beta ; cleft
    Vědní obor RIVEB - Genetika a molekulární biologie
    Obor OECDCell biology
    CEPGA13-20293S GA ČR - Grantová agentura ČR
    LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaUMG-J - RVO:68378050
    UT WOS000413398800003
    DOI10.3892/ijmm.2017.3128
    AnotaceThe nonsyndromic cleft is one of the most frequent congenital defects in humans. Clinical data demonstrated improved and almost scarless neonatal healing of reparative surgery. Based on our previous results on crosstalk between neonatal fibroblasts and adult keratinocytes, the present study focused on characterization of fibroblasts prepared from cleft lip tissue samples of neonates and older children, and compared them with samples isolated from normal adult skin (face and breast) and scars. Although subtle variances in expression profiles of children and neonates were observed, the two groups differed significantly from adult cells. Compared with adult cells, differences were observed in nestin and smooth muscle actin (SMA) expression at the protein and transcript level. Furthermore, fibroblast to myofibroblast differentiation drives effective wound healing and is largely regulated by the cytokine, transforming growth factor-beta 1 (TGF-beta 1). Dysregulation of the TGF-beta signalling pathway, including low expression of the TGF-beta receptor II, may contribute to reducing scarring in neonates. Fibroblasts of facial origin also exhibited age independent differences from the cells prepared from the breast, reflecting the origin of the facial cells from neural crest-based ectomesenchyme.
    PracovištěÚstav molekulární genetiky
    KontaktNikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
    Rok sběru2018
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.