Počet záznamů: 1

Enzyme activity of topsoii layer on reclaimed and unreclaimed post-mining sites

SYSNO ASEP	0486225
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Ostatní články
Název	Enzyme activity of topsoii layer on reclaimed and unreclaimed post-mining sites
Tvůrce(i)	Heděnec, Petr (BC-A) Vindušková, O. (CZ) Kukla, J. (CZ) Šnajdr, Jaroslav (MBU-M) Baldrian, Petr (MBU-M)_{RID, ORCID} Frouz, Jan (BC-A)_{RID, ORCID}
Zdroj.dok.	Biological Communications - ISSN 2542-2154 Roč. 62, č. 1 (2017), s. 19-25
Poč.str.	7 s.
Jazyk dok.	eng - angličtina
Země vyd.	RU - Rusko
Klíč. slova	enzyme assay ; microbial activity ; litterbag ; macrofauna ; soil fauna
Vědní obor RIV	DF - Pedologie
Obor OECD	Soil science
Vědní obor RIV – spolupráce	Mikrobiologický ústav - Mikrobiologie, virologie
CEP	LC06066 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GAP504/12/1288 GA ČR - Grantová agentura ČR
Institucionální podpora	BC-A - RVO:60077344 ; MBU-M - RVO:61388971
DOI	10.21638/11701/spbu03.2017.103
Anotace	Topsoii layer contains various components of soil organic matter such as branches, leaves, bark, or metabolites and residues of soil biota. Soil organic matter (SOM) in forest ecosystems consists mostly of lignin, cellulose, chitin and other hydrocarbons. These compounds are decomposed mainly by soil fungi which produce extracellular enzymes to decompose wide range of organic residues. These enzymes may alter nutrient cycling and change soil properties such as water retention capacity, ion exchange capacity, formation of soil microstructure, soil microbial respiration, etc. In this study, we studied enzyme activity in the topsoii layer of post-mining spoil heaps near Sokolov, Czech Republic. We investigated the effect of the following factors and their combinations on enzyme activity: i) dominant vegetation, ii) time of sampling, iii) reclamation, and iv) soil macrofauna. We measured enzyme activity in plastic mesocosms with autochthonous litter deposited on reclaimed and unreclaimed post-mining sites. We used mesocosms accessible for macrofauna (mesh size >2 mm) and mesocosms inaccessible for macrofauna (mesh size<2 mm). Under laboratory conditions, we measured enzyme activity (laccase, oxidase, peroxidase, Mn-peroxidase, alkaline phosphatase, acid phosphatase, endoglucanase, xylanase, cellobiohydrolase, glucosidase, xylosidase and chitinase) using buffer extraction method followed by spectrophotometric assay. We did not find any statistically significant difference between mesocosms accessible and inaccessible for fauna. However, we found significant effect of time of sampling on enzyme activity. Our results showed significant difference between reclaimed and unreclaimed post-mining sites. Reclaimed sites showed significantly higher enzyme activity than unreclaimed sites.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2018

Počet záznamů: 1