Počet záznamů: 1
gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity preliminary methodological study and discussion
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SYSNO ASEP 0485555 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity preliminary methodological study and discussion Tvůrce(i) Falk, Martin (BFU-R) RID, ORCID
Hořáková, Z. (CZ)
Svobodová, M. (CZ)
Masařík, M. (CZ)
Kopečná, Olga (BFU-R) ORCID
Gumulec, J. (CZ)
Raudenská, M. (CZ)
Depeš, Daniel (BFU-R)
Bačíková, Alena (BFU-R)
Falková, Iva (BFU-R) ORCID
Binkova, H. (CZ)Celkový počet autorů 11 Číslo článku 241 Zdroj.dok. European Physical Journal D. - : Springer - ISSN 1434-6060
Roč. 71, č. 9 (2017)Poč.str. 7 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova squamous-cell carcinoma ; cancer-associated fibroblasts Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP GA16-12454S GA ČR - Grantová agentura ČR Institucionální podpora BFU-R - RVO:68081707 UT WOS 000411083200015 DOI https://doi.org/10.1140/epjd/e2017-80073-2 Anotace In order to improve patients' post-treatment quality of life, a shift from surgery to non-surgical (chemo) radio-treatment is recognized in head and neck oncology. However, about half of HNSCC tumors are resistant to irradiation and an efficient marker of individual tumor radiosensitivity is still missing. We analyzed whether various parameters of DNA double strand break (DSB) repair determined in vitro can predict, prior to clinical treatment initiation, the radiosensitivity of tumors. We compared formation and decrease of gamma H2AX/53BP1 foci in 48 h after irradiating tumor cell primocultures with 2 Gy of gamma-rays. To better understand complex tumor behavior, three different cell type primocultures CD90(-), CD90(+), and a mixed culture of these cells were isolated from 1 clinically radioresistant, 2 radiosensitive, and 4 undetermined HPV-HNSCC tumors and followed separately. While DSB repair was delayed and the number of persisting DSBs increased in the radiosensitive tumors, the results for the radioresistant tumor were similar to cultured normal human skin fibroblasts. Hence, DSB repair kinetics/efficiency may correlate with clinical response to radiotherapy for a subset of HNSCC tumors but the size (and therefore practical relevance) of this subset remains to be determined. The same is true for contribution of different cell type primocultures to tumor radioresistance. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2018
Počet záznamů: 1