Počet záznamů: 1
Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research
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SYSNO ASEP 0485263 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research Tvůrce(i) Kupcová Skalníková, Helena (UZFG-Y) RID, ORCID
Čížková, Jana (UZFG-Y)
Červenka, Jakub (UZFG-Y) ORCID
Vodička, Petr (UZFG-Y) ORCIDČíslo článku 2697 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 18, č. 12 (2017)Poč.str. 32 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova cytokine ; cancer ; melanoma ; secretome ; proteomics Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemical research methods CEP GA16-05534S GA ČR - Grantová agentura ČR LO1609 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000418896700199 EID SCOPUS 85049870560 DOI 10.3390/ijms18122697 Anotace Melanoma is a skin cancer with permanently increasing incidence and resistance to therapies in advanced stages. Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. Cytokines are key factors regulating immune response and intercellular communication in tumour microenvironment. Cytokines may be used in therapy of melanoma to modulate immune response. Cytokines also possess diagnostic and prognostic potential and cytokine production may reflect effects of immunotherapies. The purpose of this review is to give an overview of recent advances in proteomic techniques for the detection and quantification of cytokines in melanoma research. Approaches covered span from mass spectrometry to immunoassays for single molecule detection (ELISA, western blot), multiplex assays (chemiluminescent, bead-based (Luminex) and planar antibody arrays), ultrasensitive techniques (Singulex, Simoa, immuno-PCR, proximity ligation/extension assay, immunomagnetic reduction assay), to analyses of single cells producing cytokines (ELISpot, flow cytometry, mass cytometry and emerging techniques for single cell secretomics). Although this review is focused mainly on cancer and particularly melanoma, the discussed techniques are in general applicable to broad research field of biology and medicine, including stem cells, development, aging, immunology and intercellular communication. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2018
Počet záznamů: 1