Počet záznamů: 1
Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization
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SYSNO ASEP 0481790 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization Tvůrce(i) Krajčovič, T. (SK)
Bučko, M. (SK)
Vikartovská, A. (SK)
Lacík, I. (SK)
Uhelská, L. (SK)
Chorvát, D. (SK)
Neděla, Vilém (UPT-D) RID, ORCID, SAI
Tihlaříková, Eva (UPT-D) RID, ORCID, SAI
Gericke, M. (DE)
Heinze, T. (DE)
Gemeiner, P. (SK)Celkový počet autorů 11 Zdroj.dok. Catalysts. - : MDPI
Roč. 7, č. 11 (2017), s. 353-364Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova polyelectrolyte complex beads ; environmental scanning electron microscopy ; confocal laser scanning microscopy ; Baeyer-Villiger biooxidation ; cyclohexanone monoxygenase ; immobilization ; viable whole-cell biocatalyst Vědní obor RIV JA - Elektronika a optoelektronika, elektrotechnika Obor OECD Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation Institucionální podpora UPT-D - RVO:68081731 UT WOS 000416780900042 EID SCOPUS 85035245420 DOI 10.3390/catal7110353 Anotace A novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells exhibited higher operational stability than free cells during 10 repeated cycles of Baeyer–Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to the corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one. The morphology of polyelectrolyte complex beads was characterised by environmental scanning electron microscopy, the spatial distribution of polymers in the beads and cell viability were examined using confocal laser scanning microscopy, and the texture was characterised by the mechanical resistance measurements. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2018 Elektronická adresa http://www.mdpi.com/2073-4344/7/11/353
Počet záznamů: 1