Počet záznamů: 1
Selective rab11 transport and the intrinsic regenerative ability of CNS axons
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SYSNO ASEP 0481164 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Selective rab11 transport and the intrinsic regenerative ability of CNS axons Tvůrce(i) Koseki, H. (GB)
Donegá, M. (GB)
Lam, B.Y.H. (GB)
Petrová, V. (GB)
van Erp, S. (GB)
Yeo, G.S.H. (GB)
Kwok, Jessica (UEM-P) ORCID, RID
Ffrench-Constant, Ch. (GB)
Eva, R. (GB)
Fawcett, James (UEM-P) ORCIDZdroj.dok. eLife. - : eLife - ISSN 2050-084X
Roč. 6, aug (2017), e26956Poč.str. 25 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova spinal-cord-injury ; chondroitin sulfate proteoglycans ; growth cone Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy Obor OECD Neurosciences (including psychophysiology CEP EF15_003/0000419 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 UT WOS 000411664100001 EID SCOPUS 85030710450 DOI 10.7554/eLife.26956 Anotace Neurons lose intrinsic axon regenerative ability with maturation, but the mechanism remains unclear. Using an in-vitro laser axotomy model, we show a progressive decline in the ability of cut CNS axons to form a new growth cone and then elongate. Failure of regeneration was associated with increased retraction after axotomy. Transportation into axons becomes selective with maturation, we hypothesized that selective exclusion of molecules needed for growth may contribute to regeneration decline. With neuronal maturity rab11 vesicles (which carry many molecules involved in axon growth) became selectively targeted to the somatodendritic compartment and excluded from axons by predominant retrograde transport However, on overexpression rab11 was mistrafficked into proximal axons, and these axons showed less retraction and enhanced regeneration after axotomy. These results suggest that the decline of intrinsic axon regenerative ability is associated with selective exclusion of key molecules, and that manipulation of transport can enhance regeneration. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2018
Počet záznamů: 1