Počet záznamů: 1
Diagnostic techniques for viroids
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SYSNO ASEP 0479353 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Diagnostic techniques for viroids Tvůrce(i) Gucek, T. (SI)
Trdan, S. (SI)
Jakše, J. (SI)
Javornik, B. (SI)
Matoušek, Jaroslav (BC-A) RID, ORCID
Radisek, S. (SI)Celkový počet autorů 6 Zdroj.dok. Plant Pathology. - : Wiley - ISSN 0032-0862
Roč. 66, č. 3 (2017), s. 339-358Poč.str. 15 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova potato-spindle-tuber ; multiplex rt-pcr ; mediated isothermal amplification Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Biochemistry and molecular biology Institucionální podpora BC-A - RVO:60077344 UT WOS 000398606200001 EID SCOPUS 84997173313 DOI 10.1111/ppa.12624 Anotace The fast growth of the human population forces us to produce more food, but higher crop production also leads to the fast spread of diseases. Plant pathology deploys a wide range of methods that do not provide an adequate solution to all disease losses. In the case of viroids, therapeutic means of control are not available, therefore control strategies are more focused on the development of reliable detection methods to quickly exclude the infected plant material. Although viroids are the smallest and simplest plant pathogens, their identification and detection is not straightforward. Each viroid-host combination is specific, and for reliable identification, all steps from sampling to final detection must be performed accurately. In this review, several methods for viroid detection in various host plants are discussed, including their advantages and disadvantages. Even though relatively new molecular methods enable fast and sensitive detection of viroids, a combination of different methods gives the most reliable identification. Techniques based on nucleic acids may be the future for viroid detection but they still cannot replace biological indexing, which is usually essential in epidemiological and aetiological studies. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2018
Počet záznamů: 1