Počet záznamů: 1

The intermembrane space protein Erv1 of Trypanosoma brucei is essential for mitochondrial Fe-S cluster assembly and operates alone

SYSNO ASEP	0479062
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	The intermembrane space protein Erv1 of Trypanosoma brucei is essential for mitochondrial Fe-S cluster assembly and operates alone
Tvůrce(i)	Haindrich, Alexander C. (BC-A) Boudova, M. (CZ) Vancová, Marie (BC-A)_{RID, ORCID} Peña-Diaz, Priscila (BC-A)_{ORCID, RID} Horáková, Eva (BC-A)_{RID, ORCID} Lukeš, Julius (BC-A)_{RID, ORCID}
Celkový počet autorů	6
Zdroj.dok.	Molecular and Biochemical Parasitology. - : Elsevier - ISSN 0166-6851 Roč. 214, JUN (2017), s. 47-51
Poč.str.	5 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Trypanosoma ; Erv1 ; Fe-S cluster assembly ; mitochondrion
Vědní obor RIV	EB - Genetika a molekulární biologie
Obor OECD	Biochemistry and molecular biology
CEP	GA15-21974S GA ČR - Grantová agentura ČR
	GA16-18699S GA ČR - Grantová agentura ČR
Institucionální podpora	BC-A - RVO:60077344
UT WOS	000404795200006
EID SCOPUS	85017144077
DOI	10.1016/j.molbiopara.2017.03.009
Anotace	Sulfhydryl oxidase Erv1 is a ubiquitous and conserved protein of the mitochondrial intermembrane space that plays a role in the transport of small sulfur-containing proteins. In higher eukaryotes, Erv1 interacts with the mitochondrial import protein Mia40. However, Trypanosoma brucei lacks an obvious Mia40 homologue in its genome. Here we show by tandem affinity purification and mass spectrometry that in this excavate protist, Erv1 functions without a Mia40 homologue and most likely any other interaction partner. Down-regulation of TbErvl caused a reduction of the mitochondrial membrane potential already within 24 h to less than 50% when compared with control cells. The depletion of TbErv1 was accompanied by accumulation of trCOIV precursor, with a concomitant reduction of aconitase activity both in the cytosol and mitochondrion. Overall, TbErv1 seems to have a role in the mitochondrial translocation and Fe-S cluster assembly in the organelle. (C) 2017 Elsevier B.V. All rights reserved.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2018

Počet záznamů: 1