Počet záznamů: 1

Determination of effective charges and ionic mobilities of polycationic antimicrobial peptides by capillary isotachophoresis and capillary zone electrophoresis

SYSNO ASEP	0478419
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Determination of effective charges and ionic mobilities of polycationic antimicrobial peptides by capillary isotachophoresis and capillary zone electrophoresis
Tvůrce(i)	Tůmová, Tereza (UOCHB-X)_RID Monincová, Lenka (UOCHB-X) Nešuta, Ondřej (UOCHB-X)_{RID, ORCID} Čeřovský, Václav (UOCHB-X)_{RID, ORCID} Kašička, Václav (UOCHB-X)_{RID, ORCID}
Zdroj.dok.	Electrophoresis. - : Wiley - ISSN 0173-0835 Roč. 38, č. 16 (2017), s. 2018-2024
Poč.str.	7 s.
Jazyk dok.	eng - angličtina
Země vyd.	DE - Německo
Klíč. slova	antimicrobial peptides ; capillary isotachophoresis ; counterion condensation ; effective charge ; ionic mobility
Vědní obor RIV	CB - Analytická chemie, separace
Obor OECD	Analytical chemistry
CEP	GA15-01948S GA ČR - Grantová agentura ČR
Institucionální podpora	UOCHB-X - RVO:61388963
UT WOS	000408038500006
EID SCOPUS	85019765806
DOI	10.1002/elps.201700092
Anotace	Capillary ITP (CITP) and CZE were applied to the determination of effective charges and ionic mobilities of polycationic antimicrobial peptides (AMPs). Twelve AMPs (deca- to hexadecapeptides) containing three to seven basic amino acid residues (His, Lys, Arg) at variable positions of peptide chain were investigated. Effective charges of the AMPs were determined from the lengths of their ITP zones, ionic mobilities, and molar concentrations, and from the same parameters of the reference compounds. Lengths of the ITP zones of AMPs and reference compounds were obtained from their CITP analyses in cationic mode using leading electrolyte (LE) composed of 10mMNH(4)OH, 40mMAcOH (acetic acid), pH 4.1, and terminating electrolyte (TE) containing 40mMAcOH, pH 3.2. Ionic mobilities of AMPs and singly charged reference compounds (ammediol or arginine) were determined by their CZE analyses in the BGE of the same composition as the LE. The effective charges numbers of AMPs were found to be in the range 1.65-5.04, i.e. significantly reduced as compared to the theoretical charge numbers (2.86-6.99) calculated from the acidity constants of the analyzed AMPs. This reduction of effective charge due to tightly bound acetate counterions (counterion condensation) was in the range 17-47% depending on the number and type of the basic amino acid residues in the AMPs molecules. Ionic mobilities of AMPs achieved values (26.5-38.6)x10(-9)m(2)V(-1)s(-1) and in most cases were in a good agreement with the ratio of their effective charges and relative molecular masses.
Pracoviště	Ústav organické chemie a biochemie
Kontakt	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Rok sběru	2018

Počet záznamů: 1