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Axon tension regulates fasciculation/defasciculation through the control of axon shaft zippering
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SYSNO ASEP 0476688 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Axon tension regulates fasciculation/defasciculation through the control of axon shaft zippering Tvůrce(i) Šmít, Daniel (FGU-C)
Fouquet, C. (FR)
Pincet, F. (FR)
Zápotocký, Martin (FGU-C) RID, ORCID
Trembleau, A. (FR)Číslo článku e19907 Zdroj.dok. eLife. - : eLife - ISSN 2050-084X
Roč. 6, Apr 19 (2017)Poč.str. 49 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova biophysics ; cell adhesion ; coarsening ; developmental biology ; mathematical model ; mechanical tension ; axon guidance Vědní obor RIV BO - Biofyzika Obor OECD Biophysics CEP GA14-16755S GA ČR - Grantová agentura ČR 7AMB12FR002 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora FGU-C - RVO:67985823 UT WOS 000403786800001 EID SCOPUS 85021442510 DOI 10.7554/eLife.19907 Anotace While axon fasciculation plays a key role in the development of neural networks, very little is known about its dynamics and the underlying biophysical mechanisms. In a model system composed of neurons grown ex vivo from explants of embryonic mouse olfactory epithelia, we observed that axons dynamically interact with each other through their shafts, leading to zippering and unzippering behavior that regulates their fasciculation. Taking advantage of this new preparation suitable for studying such interactions, we carried out a detailed biophysical analysis of zippering, occurring either spontaneously or induced by micromanipulations and pharmacological treatments. We show that zippering arises from the competition of axon-axon adhesion and mechanical tension in the axons, and provide the first quantification of the force of axon-axon adhesion. Furthermore, we introduce a biophysical model of the zippering dynamics, and we quantitatively relate the individual zipper properties to global characteristics of the developing axon network. Our study uncovers a new role of mechanical tension in neural development: the regulation of axon fasciculation. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2018
Počet záznamů: 1