Počet záznamů: 1

Detection of herbicide effects on pigment composition and PSII photochemistry in Helianthus annuus by Raman spectroscopy and chlorophyll a fluorescence

SYSNO ASEP	0473958
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Detection of herbicide effects on pigment composition and PSII photochemistry in Helianthus annuus by Raman spectroscopy and chlorophyll a fluorescence
Tvůrce(i)	Vítek, Petr (UEK-B)_{RID, SAI} Surá, Kateřina (UEK-B)_{RID, SAI, ORCID} Hodaňová, Petra (UEK-B)_{SAI, RID} Rapantová, Barbora (UEK-B)_{RID, SAI} Klem, Karel (UEK-B)_{RID, ORCID, SAI}
Zdroj.dok.	Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. - : Elsevier - ISSN 1386-1425 Roč. 170, Jan (2017), s. 234-241
Poč.str.	8 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Raman mapping ; Chlorophyll fluorescence ; Carotenoids ; Flavonoids ; Photosynthesis ; Secondary metabolites
Vědní obor RIV	EH - Ekologie - společenstva
Obor OECD	Environmental sciences (social aspects to be 5.7)
CEP	LO1415 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	QJ1530373 GA MZe - Ministerstvo zemědělství
Výzkumná infrastruktura	CzeCOS II - 90061 - Ústav výzkumu globální změny AV ČR, v. v. i.
Institucionální podpora	RVO:67179843 - RVO:67179843
UT WOS	000398746600031
EID SCOPUS	84981747705
DOI	10.1016/j.saa.2016.07.025
Anotace	The effects of herbicides from three mode-of-action groups – inhibitors of protoporphyrinogen oxidase (carfentrazone-ethyl), inhibitors of carotenoid biosynthesis (mesotrione, clomazone, and diflufenican), and inhibitors of acetolactate synthase (amidosulfuron) – were studied in sunflower plants (Helianthus annuus). Raman spectroscopy, chlorophyll fluorescence (ChlF) imaging, and UV screening of ChlF were combined to evaluate changes in pigment composition, photosystem II (PSII) photochemistry, and non-photochemical quenching in plant leaves 6 d after herbicide application. The Raman signals of phenolic compounds, carotenoids, and chlorophyll were evaluated and differences in their intensity ratios were observed. Strongly augmented relative content of phenolic compounds was observed in the case of amidosulfuron-treated plants, with a simultaneous decrease in the chlorophyll/carotenoid intensity ratio. The results were confirmed by in vivo measurement of flavonols using UV screening of ChlF. Herbicides from the group of carotenoid biosynthesis inhibitors significantly decreased both the maximum quantum efficiency of PSII and non-photochemical quenching as determined by ChlF. Resonance Raman imaging (mapping) data with high resolution (150,000–200,000 spectra) are presented, showing the distribution of carotenoids in H. annuus leaves treated by two of the herbicides acting as inhibitors of carotenoid biosynthesis (clomazone or diflufenican). Clear signs were observed that the treatment induced carotenoid depletion within sunflower leaves. The depletion spatial pattern registered differed depending on the type of herbicide applied.
Pracoviště	Ústav výzkumu globální změny
Kontakt	Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268
Rok sběru	2018

Počet záznamů: 1