Počet záznamů: 1
Recent advances and challenges in the heterologous production of microbial nitrilases for biocatalytic applications
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SYSNO ASEP 0473528 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Recent advances and challenges in the heterologous production of microbial nitrilases for biocatalytic applications Tvůrce(i) Martínková, Ludmila (MBU-M) RID, ORCID
Rucká, Lenka (MBU-M)
Nešvera, Jan (MBU-M) RID
Pátek, Miroslav (MBU-M) RID, ORCIDČíslo článku 8 Zdroj.dok. World Journal of Microbiology and Biotechnology. - : Springer - ISSN 0959-3993
Roč. 33, č. 1 (2017), s. 1-11Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Aldoxime-nitrile pathway ; Biocatalytic applications ; Database mining Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP LD15107 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000392115600008 EID SCOPUS 84995772487 DOI 10.1007/s11274-016-2173-6 Anotace The aim of this study is to review the current state of and highlight the challenges in the production of microbial nitrilases as catalysts for the mild hydrolysis of industrially important nitriles. Together with aldoxime dehydratase, the nitrile-hydrolyzing enzymes (nitrilase, nitrile hydratase) are key enzymes in the aldoxime-nitrile pathway which is widely distributed in bacteria and fungi. The availability of nitrilases has grown significantly over the past decade due to the use of metagenomic and database-mining approaches. Databases contain plenty of putative enzymes of this type, whose overproduction may improve the spectrum and the industrial utility of nitrilases. By exploiting this resource, the number of experimentally verified nitrilases has recently increased to several hundred. We especially focus on the efficient heterologous expression systems that are applicable for the overproduction of wild-type nitrilases and their artificial variants. Biocatalyst forms with industrial potential are also highlighted. The potential industrial applications of nitrilases are classified according to their target products (a-hydroxy acids, a-and beta-amino acids, cyano acids, amides). The emerging uses of nitrilases and their subtypes (cyanide hydratases, cyanide dihydratases) in bioremediation is also summarized. The integration of nitrilases with other enzymes into artificial multienzymatic and chemoenzymatic pathways is considered a promising strategy for future applications. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
Počet záznamů: 1