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BFPTool: a software tool for analysis of Biomembrane Force Probe experiments
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SYSNO ASEP 0471014 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název BFPTool: a software tool for analysis of Biomembrane Force Probe experiments Tvůrce(i) Šmít, Daniel (FGU-C)
Fouquet, C. (FR)
Doulazmi, M. (FR)
Pincet, F. (FR)
Trembleau, A. (FR)
Zápotocký, Martin (FGU-C) RID, ORCIDČíslo článku 2 Zdroj.dok. BMC Biophysics
Roč. 10, Feb (2017)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Biomembrane Force Probe ; motion tracking ; image analysis ; force spectroscopy ; cell mechanics Vědní obor RIV BO - Biofyzika Obor OECD Biophysics CEP GA14-16755S GA ČR - Grantová agentura ČR 7AMB12FR002 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora FGU-C - RVO:67985823 UT WOS 000397045500001 EID SCOPUS 85012914208 DOI 10.1186/s13628-016-0033-2 Anotace The Biomembrane Force Probe is an approachable experimental technique commonly used for single-molecule force spectroscopy and experiments on biological interfaces. The technique operates in the range of forces from 0.1 pN to 1000 pN. Experiments are typically repeated many times, conditions are often not optimal, the captured video can be unstable and lose focus; this makes efficient analysis challenging, while out-of-the-box non-proprietary solutions are not freely available.This dedicated tool was developed to integrate and simplify the image processing and analysis of videomicroscopy recordings from BFP experiments. A novel processing feature, allowing the tracking of the pipette, was incorporated to address a limitation of preceding methods. Emphasis was placed on versatility and comprehensible user interface implemented in a graphical form.An integrated analytical tool was implemented to provide a faster, simpler and more convenient way to process and analyse BFP experiments. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2017
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