Počet záznamů: 1
Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta)
- 1.
SYSNO ASEP 0468874 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta) Tvůrce(i) Jerez, C.G. (ES)
Malapascua, José R. (MBU-M)
Sergejevová, Magda (MBU-M)
Figueroa, Felix L. (ES)
Masojídek, Jiří (MBU-M) RID, ORCIDZdroj.dok. Marine Biotechnology - ISSN 1436-2228
Roč. 18, č. 1 (2016), s. 24-36Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Microalgae ; Chlorella ; Lipid Vědní obor RIV EE - Mikrobiologie, virologie CEP ED2.1.00/03.0110 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EE2.3.30.0059 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EE2.3.20.0203 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000378136600003 EID SCOPUS 84953354282 DOI 10.1007/s10126-015-9664-6 Anotace The effect of nitrogen and sulphur limitation under high irradiance (PAR) was studied in the green microalga Chlorella fusca (Chlorophyta) in order to follow lipid and/or starch accumulation. Growth, biomass composition and the changes in photosynthetic activity (in vivo chlorophyll a fluorescence) were followed in the trials. The full nutrient culture showed high biomass production and starch accumulation at Day 1, when photosynthetic activity was high. Gradual deprivation (no nutrients added) became evident when photosynthesis was significantly suppressed (Day 3 onwards), which entailed a decrease of maximum relative electron transport rate (rETR(max)) and increase of non-photochemical quenching (NPQ), accompanied by the onset of lipid accumulation and decline in starch content. In N- and S-starved cultures, rETRmax significantly decreased by Day 3, which caused a substantial drop in biomass production, cell number, biovolume and induction of lipid and starch accumulation. High starch content (45-50 % of DW) was found at the initial stage in full nutrient culture and at the stationary phase in nutrient-starved cultures. By the end of the trial, all treatments showed high lipid content (similar to 30 % of DW). The full nutrient culture had higher biomass yield than starved treatments although starch (similar to 0.2 g L-1 day(-1)) and lipid (similar to 0.15 g L-1 day(-1)) productivities were fairly similar in all the cultures. Our results showed that we could enrich biomass of C. fusca (%DW) in lipids using a two-stage strategy (a nutrient replete stage followed by gradual nutrient limitation) while under either procedure, N- or S-starvation, both high lipid and starch contents could be achieved. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
Počet záznamů: 1