Počet záznamů: 1
Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation
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SYSNO ASEP 0468729 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation Tvůrce(i) Dzyuba, V. (CZ)
Słowińska, M. (PL)
Cosson, J. (CZ)
Ciereszko, A. (PL)
Boryshpolets, S. (CZ)
Štěrba, Ján (BC-A) RID, ORCID
Rodina, M. (CZ)
Linhart, O. (CZ)
Dzyuba, B. (CZ)Celkový počet autorů 9 Zdroj.dok. Fish Physiology and Biochemistry. - : Springer - ISSN 0920-1742
Roč. 42, č. 6 (2016), s. 1755-1766Poč.str. 12 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova amidase activity ; anti-proteolytic activity ; casein and gelatin zymography ; siberian sturgeon sperm ; spermatozoon maturation ; sterlet sperm Vědní obor RIV ED - Fyziologie CEP EE2.3.30.0032 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BC-A - RVO:60077344 UT WOS 000389407900021 EID SCOPUS 84978116144 DOI 10.1007/s10695-016-0255-x Anotace In sturgeon, the acquisition of the potential for motility activation called spermatozoon maturation takes place outside testes. This process can be accomplished in vitro by pre-incubation of immature testicular spermatozoa in seminal fluid collected from fully mature Wolffian duct sperm. Addition of trypsin inhibitor to the pre-incubation medium disrupts spermatozoon maturation. There are no available data for the role of proteolysis regulators in fish spermatozoon maturation, while their role is recognized in mammalian sperm maturation. The present study evaluated the involvement of seminal fluid proteases and anti-proteolytic activity in the sterlet spermatozoon maturation process. Casein and gelatin zymography and quantification of amidase and anti-proteolytic activity were conducted in sturgeon seminal fluid from Wolffian duct sperm and seminal fluid from testicular sperm, along with spermatozoon extracts from Wolffian duct spermatozoa, testicular spermatozoa, and testicular spermatozoa after in vitro maturation. We did not find significant differences in proteolytic profiles of seminal fluids from Wolffian duct sperm and ones from testicular sperm. Zymography revealed differences in spermatozoon extracts: Wolffian duct spermatozoon extracts were characterized by the presence of a broad proteolytic band ranging from 48 to 41 kDa, while testicular spermatozoon extracts did not show such activity until after in vitro maturation. The differences in amidase activity coincided with these results. It may not be the levels of proteolytic and anti-proteolytic activity per se, but the alterations in their interactions triggering a cascade of signaling events, that is crucial to the maturation process. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2017
Počet záznamů: 1