Počet záznamů: 1  

Dimeric cyanobacterial cyclopent-4-ene-1,3-dione as selective inhibitor of Gram-positive bacteria growth: Bio-production approach and preparative isolation by HPCCC

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    SYSNO ASEP0467891
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevDimeric cyanobacterial cyclopent-4-ene-1,3-dione as selective inhibitor of Gram-positive bacteria growth: Bio-production approach and preparative isolation by HPCCC
    Tvůrce(i) Cheel, José (MBU-M) RID, ORCID
    Bogdanová, K. (CZ)
    Ignatova, S. (GB)
    Garrard, I. (GB)
    Hewitson, P. (GB)
    Kolář, M. (CZ)
    Kopecký, Jiří (MBU-M) ORCID
    Hrouzek, Pavel (MBU-M) ORCID
    Vacek, J. (CZ)
    Zdroj.dok.Algal Research-Biomass Biofuels and Bioproducts - ISSN 2211-9264
    Roč. 18, SEP (2016), s. 244-249
    Poč.str.6 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovaPhenolic cyclopentenedione ; Natural antibiotic ; Cyanobacteria
    Vědní obor RIVEE - Mikrobiologie, virologie
    CEPEE2.3.30.0059 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    ED2.1.00/03.0110 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    LO1416 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000381748800028
    EID SCOPUS84976428074
    DOI10.1016/j.algal.2016.06.022
    AnotaceThe need for new antimicrobial agents is greater than ever because of the emergence of multidrug resistance in common pathogens and incidence of new infections. Cyclopent-4-ene-1,3-diones (CPDs) have been reported as a new class of compounds with promising antimicrobial and antifungal properties. Herein we report the selective antibiotic properties of nostotrebin 6, a phenolic CPD produced biotechnologically by the culture of cyanobacterium Nostoc sp. str. Lukesova 27/97. High performance countercurrent chromatography (HPCCC) combined with gel permeation chromatography (GPC) was used for the isolation of nostotrebin 6 with a relatively high 0.53 +/- 0.1% yield (calculated from dried biomass) and final purity higher than 96%. Nostotrebin 6 was tested for its antimicrobial and antifungal activities by using standard micro-dilution method, and the results were expressed as minimal inhibitory concentrations (MICs). Nostotrebin 6 unequivocally inhibited the growth of Gram-positive reference (Enterococcus faecalis CCM 4224, Staphylococcus aureus CCM 4223 and Staphylococcus aureus CCM 3953) and multidrug-resistant (Staphylococcus haemolyticus A/16568, Staphylococcus aureus MRSA 4591 and Enterococcus faecium VanA 419/ana) strains. Its strongest effect was exerted against the Gram-positive bacteria with MICs ranging between 6.25 and 15.6 mu g/mL. There was no effect on Gram-negative strains tested and yeasts. Our results suggest that nostotrebin 6 could serve as basic nucleus for further design of novel antibiotic agents and demonstrate that the bio-production approach based on HPCCC/GPC isolation endpoint is an efficient methodology for obtaining nostotrebin 6 in multi-gram scale. Furthermore, the presented isolation method can be easily up-scaled to process kilograms of the cyanobacterial biomass.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2017
Počet záznamů: 1