Počet záznamů: 1  

Effects of transgenic expression of dopamine beta hydroxylase (Dbh) gene on blood pressure in spontaneously hypertensive rats

  1. 1.
    SYSNO ASEP0467648
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevEffects of transgenic expression of dopamine beta hydroxylase (Dbh) gene on blood pressure in spontaneously hypertensive rats
    Tvůrce(i) Pravenec, Michal (FGU-C) RID, ORCID
    Landa, Vladimír (FGU-C) RID
    Zídek, Václav (FGU-C) RID
    Mlejnek, Petr (FGU-C) RID, ORCID
    Šilhavý, Jan (FGU-C) RID, ORCID
    Mir, S.A. (US)
    Vaingankar, S. M. (US)
    Wang, J. (US)
    Kurtz, T. W. (US)
    Zdroj.dok.Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
    Roč. 65, č. 6 (2016), s. 1039-1044
    Poč.str.6 s.
    Jazyk dok.eng - angličtina
    Země vyd.CZ - Česká republika
    Klíč. slovaspontaneously hypertensive rat ; transgenic ; dopamine beta hydroxylase ; catecholamines ; blood pressure ; left ventricular mass
    Vědní obor RIVFB - Endokrinologie, diabetologie, metabolizmus, výživa
    CEPGAP301/12/0696 GA ČR - Grantová agentura ČR
    TA02010013 GA TA ČR - Technologická agentura ČR
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000391198200015
    EID SCOPUS85011844896
    AnotaceThe spontaneously hypertensive rat (SHR) is the most widely used animal model of essential hypertension and left ventricular hypertrophy. Catecholamines play an important role in the pathogenesis of both essential hypertension in humans and in the SHR. Recently, we obtained evidence that the SHR harbors a variant in the gene for dopamine beta hydroxylase (Dbh) that is associated with reduced adrenal expression of Dbh mRNA and reduced DBH enzymatic activity which correlated negatively with blood pressure. In the current study, we used a transgenic experiment to test the hypothesis that reduced Dbh expression predisposes the SHR to hypertension and that augmentation of Dbh expression would reduce blood pressure. We derived 2 new transgenic SHR-Dbh lines expressing Dbh cDNA under control of the Brown Norway (BN) wild type promoter. We found modestly increased adrenal expression of Dbh in transgenic rats versus SHR non-transgenic controls that was associated with reduced adrenal levels of dopamine and increased plasma levels of norepinephrine and epinephrine. The observed changes in catecholamine metabolism were associated with increased blood pressure and left ventricular mass in both transgenic lines. We did not observe any consistent changes in brainstem levels of catecholamines or of mRNA levels of Dbh in the transgenic strains. Contrary to our initial expections, these findings are consistent with the possibility that genetically determined decreases in adrenal expression and activity of DBH do not represent primary determinants of increased blood pressure in the SHR model.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2017
Počet záznamů: 1  

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