Počet záznamů: 1  

Interactions of helquats with chiral acidic aromatic analytes investigated by partial-filling affinity capillary electrophoresis

  1. 1.
    SYSNO ASEP0466571
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevInteractions of helquats with chiral acidic aromatic analytes investigated by partial-filling affinity capillary electrophoresis
    Tvůrce(i) Růžička, Martin (UOCHB-X) RID
    Koval, Dušan (UOCHB-X) RID, ORCID
    Vávra, Jan (UOCHB-X) RID
    Reyes Gutierrez, Paul Eduardo (UOCHB-X) RID, ORCID
    Teplý, Filip (UOCHB-X) RID, ORCID
    Kašička, Václav (UOCHB-X) RID, ORCID
    Zdroj.dok.Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
    Roč. 1467, Oct 7 (2016), s. 417-426
    Poč.str.10 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovaaffinity capillary electrophoresis ; binding constant ; chiral separation ; helquats ; noncovalent interactions ; partial filling
    Vědní obor RIVCB - Analytická chemie, separace
    CEPGA15-01948S GA ČR - Grantová agentura ČR
    GA13-32974S GA ČR - Grantová agentura ČR
    GA13-19213S GA ČR - Grantová agentura ČR
    Institucionální podporaUOCHB-X - RVO:61388963
    UT WOS000385323800040
    EID SCOPUS84991054850
    DOI10.1016/j.chroma.2016.08.053
    AnotaceNoncovalent molecular interactions between helquats, a new class of dicationic helical extended diquats, and several chiral acidic aromatic drugs and catalysts have been investigated using partial-filling affinity capillary electrophoresis (PF-ACE). Helquats dissolved at 1 mM concentration in the aqueous background electrolyte (40 mM Tris, 20 mM acetic acid, pH 8.1) were introduced as ligand zones of variable length (0-130 mm) into the hydroxypropylcellulose coated fused silica capillary whereas 0.1 mM solutions of negatively charged chiral drugs or catalysts (warfarin, ibuprofen, mandelic acid, etodolac, binaphthyl phosphate and 11 other acidic aromatic compounds) were applied as a short analyte zone at the injection capillary end. After application of electric field, analyte and ligand migrated against each other and in case of their interactions, migration time of the analyte was increasing with increasing length of the ligand zone. From the tested compounds, only isomers of those exhibiting helical chirality and/or possessing conjugated aromatic systems were enantioselectively separated through their differential interactions with helquats. Some compounds with conjugated aromatic groups interacted with helquats moderately strongly but non-enantiospecifically. Small compounds with single benzene ring exhibited no or very weak non-enantiospecific interactions. PF-ACE method allowed to determine binding constants of the analyte-helquat complexes from the changes of migration times of the analytes. Binding constants of the weakest complexes of the analytes with helquats were less than 50 L/mol, whereas binding constants of the strongest complexes were in the range 1 000-1 400 L/mol.
    PracovištěÚstav organické chemie a biochemie
    Kontaktasep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
    Rok sběru2017
Počet záznamů: 1  

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