Počet záznamů: 1
Interactions of helquats with chiral acidic aromatic analytes investigated by partial-filling affinity capillary electrophoresis
- 1.
SYSNO ASEP 0466571 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Interactions of helquats with chiral acidic aromatic analytes investigated by partial-filling affinity capillary electrophoresis Tvůrce(i) Růžička, Martin (UOCHB-X) RID
Koval, Dušan (UOCHB-X) RID, ORCID
Vávra, Jan (UOCHB-X) RID
Reyes Gutierrez, Paul Eduardo (UOCHB-X) RID, ORCID
Teplý, Filip (UOCHB-X) RID, ORCID
Kašička, Václav (UOCHB-X) RID, ORCIDZdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1467, Oct 7 (2016), s. 417-426Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova affinity capillary electrophoresis ; binding constant ; chiral separation ; helquats ; noncovalent interactions ; partial filling Vědní obor RIV CB - Analytická chemie, separace CEP GA15-01948S GA ČR - Grantová agentura ČR GA13-32974S GA ČR - Grantová agentura ČR GA13-19213S GA ČR - Grantová agentura ČR Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000385323800040 EID SCOPUS 84991054850 DOI 10.1016/j.chroma.2016.08.053 Anotace Noncovalent molecular interactions between helquats, a new class of dicationic helical extended diquats, and several chiral acidic aromatic drugs and catalysts have been investigated using partial-filling affinity capillary electrophoresis (PF-ACE). Helquats dissolved at 1 mM concentration in the aqueous background electrolyte (40 mM Tris, 20 mM acetic acid, pH 8.1) were introduced as ligand zones of variable length (0-130 mm) into the hydroxypropylcellulose coated fused silica capillary whereas 0.1 mM solutions of negatively charged chiral drugs or catalysts (warfarin, ibuprofen, mandelic acid, etodolac, binaphthyl phosphate and 11 other acidic aromatic compounds) were applied as a short analyte zone at the injection capillary end. After application of electric field, analyte and ligand migrated against each other and in case of their interactions, migration time of the analyte was increasing with increasing length of the ligand zone. From the tested compounds, only isomers of those exhibiting helical chirality and/or possessing conjugated aromatic systems were enantioselectively separated through their differential interactions with helquats. Some compounds with conjugated aromatic groups interacted with helquats moderately strongly but non-enantiospecifically. Small compounds with single benzene ring exhibited no or very weak non-enantiospecific interactions. PF-ACE method allowed to determine binding constants of the analyte-helquat complexes from the changes of migration times of the analytes. Binding constants of the weakest complexes of the analytes with helquats were less than 50 L/mol, whereas binding constants of the strongest complexes were in the range 1 000-1 400 L/mol. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2017
Počet záznamů: 1