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Protein interactions with quaternized chitosan/heparin multilayers
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SYSNO ASEP 0463595 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Protein interactions with quaternized chitosan/heparin multilayers Tvůrce(i) Kumorek, Marta M. (UMCH-V) RID
Kubies, Dana (UMCH-V) RID, ORCID
Riedel, Tomáš (UMCH-V) RID, ORCIDZdroj.dok. Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 65, Suppl. 2 (2016), S253-S261Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova heparin ; chitosan ; protein Vědní obor RIV EB - Genetika a molekulární biologie CEP LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000386686800011 EID SCOPUS 84997078065 Anotace Understanding the behavior of single proteins at the polyelectrolyte multilayer film/solution interface is of prime importance for the designing of bio-functionalized surface coatings. In the present paper, we study the adsorption of the model proteins, albumin and lysozyme, as well as basic fibroblast growth factor (FGF-2) on a polysaccharide multilayer film composed of quaternized chitosan and heparin. Several analytical methods were used to describe the formation of the polysaccharide film and its interactions with the proteins. Both albumin and lysozyme adsorbed on quaternized chitosan/heparin films, however this process strongly depended on the terminating polysaccharide. Protein adsorption was driven mainly by electrostatic interactions between protein and the terminal layer of the film. The effective binding of FGF-2 by the heparinterminated film suggested that other interactions could also contribute to the adsorption process. We believe that this FGF-2- presenting polysaccharide film may serve as a biofunctional surface coating for biologically-related applications. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2017 Elektronická adresa http://www.biomed.cas.cz/physiolres/pdf/65%20Suppl%202/65_S253.pdf
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