Počet záznamů: 1  

Protein interactions with quaternized chitosan/heparin multilayers

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    SYSNO ASEP0463595
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevProtein interactions with quaternized chitosan/heparin multilayers
    Tvůrce(i) Kumorek, Marta M. (UMCH-V) RID
    Kubies, Dana (UMCH-V) RID, ORCID
    Riedel, Tomáš (UMCH-V) RID, ORCID
    Zdroj.dok.Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
    Roč. 65, Suppl. 2 (2016), S253-S261
    Poč.str.9 s.
    Jazyk dok.eng - angličtina
    Země vyd.CZ - Česká republika
    Klíč. slovaheparin ; chitosan ; protein
    Vědní obor RIVEB - Genetika a molekulární biologie
    CEPLQ1604 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaUMCH-V - RVO:61389013
    UT WOS000386686800011
    EID SCOPUS84997078065
    AnotaceUnderstanding the behavior of single proteins at the polyelectrolyte multilayer film/solution interface is of prime importance for the designing of bio-functionalized surface coatings. In the present paper, we study the adsorption of the model proteins, albumin and lysozyme, as well as basic fibroblast growth factor (FGF-2) on a polysaccharide multilayer film composed of quaternized chitosan and heparin. Several analytical methods were used to describe the formation of the polysaccharide film and its interactions with the proteins. Both albumin and lysozyme adsorbed on quaternized chitosan/heparin films, however this process strongly depended on the terminating polysaccharide. Protein adsorption was driven mainly by electrostatic interactions between protein and the terminal layer of the film. The effective binding of FGF-2 by the heparinterminated film suggested that other interactions could also contribute to the adsorption process. We believe that this FGF-2- presenting polysaccharide film may serve as a biofunctional surface coating for biologically-related applications.
    PracovištěÚstav makromolekulární chemie
    KontaktEva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
    Rok sběru2017
    Elektronická adresahttp://www.biomed.cas.cz/physiolres/pdf/65%20Suppl%202/65_S253.pdf
Počet záznamů: 1