Počet záznamů: 1
MORN5 Expression during Craniofacial Development and Its Interaction with the BMP and TGF beta Pathways
- 1.
SYSNO ASEP 0463467 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název MORN5 Expression during Craniofacial Development and Its Interaction with the BMP and TGF beta Pathways Tvůrce(i) Celá, Petra (UZFG-Y) RID
Hampl, Marek (UZFG-Y) ORCID
Fu, K. (CA)
Kunová Bosáková, M. (CZ)
Krejčí, P. (CZ)
Richman, J. M. (CA)
Buchtová, Marcela (UZFG-Y) RID, ORCIDZdroj.dok. Frontiers in Physiology. - : Frontiers Research Foundation - ISSN 1664-042X
Roč. 7, č. 378 (2016)Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova cleft lip ; maxillary prominence ; mandibular prominence ; frontonasal mass ; BMP Vědní obor RIV EA - Morfologické obory a cytologie CEP GB14-37368G GA ČR - Grantová agentura ČR Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000382304700003 EID SCOPUS 84988723127 DOI 10.3389/fphys.2016.00378 Anotace MORN5 (MORN repeat containing 5) is encoded by a locus positioned on chromosome 17 in the chicken genome. The MORN motif is found in multiple copies in several proteins including junctophilins or phosphatidylinositol phosphate kinase family and the MORN proteins themselves are found across the animal and plant kingdoms. MORN5 protein has a characteristic punctate pattern in the cytoplasm in immunofluorescence imaging. Previously, MORN5 was found among differentially expressed genes in a microarray profiling experiment of the chicken embryo head. Here, we provided in situ hybridization to analyse, in detail, the MORN5 expression in chick craniofacial structures. The expression of MORN5 was first observed at stage HH17-18 (E2.5). MORN5 expression gradually appeared on either side of the primitive oral cavity, within the maxillary region. At stage HH20 (E3), prominent expression was localized in the mandibular prominences lateral to the midline. From stage HH20 up to HH29 (E6), there was strong expression in restricted regions of the maxillary and mandibular prominences. The frontonasal mass (in the midline of the face) expressed MORN5, starting at HH27 (E5). The expression was concentrated in the corners or globular processes, which will ultimately fuse with the cranial edges of the maxillary prominences. MORN5 expression was maintained in the fusion zone up to stage HH29. In sections MORN5 expression was localized preferentially in the mesenchyme. Previously, we examined signals that regulate MORN5 expression in the face based on a previous microarray study. We also determined that BMP pathway genes are downstream of MORN5 following siRNA knockdown. Based on these results, we conclude that MORN5 is both regulated by and required for BMP signaling. The restricted expression of MORN5 in the lip fusion zone shown here supports the human genetic data in which MORN5 variants were associated with increased risk of non-syndromic cleft lip with or without cleft palate. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2017
Počet záznamů: 1