Počet záznamů: 1

Multienzyme degradation of host serum albumin in ticks

SYSNO ASEP	0463315
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Multienzyme degradation of host serum albumin in ticks
Tvůrce(i)	Sojka, Daniel (BC-A)_{RID, ORCID} Pytelková, Jana (UOCHB-X)_RID Perner, Jan (BC-A)_{RID, ORCID} Horn, Martin (UOCHB-X)_{RID, ORCID} Konvičková, Jitka (BC-A) Schrenková, Jana (BC-A) Mareš, Michael (UOCHB-X)_{RID, ORCID} Kopáček, Petr (BC-A)_{RID, ORCID}
Zdroj.dok.	Ticks and Tick-borne Diseases - ISSN 1877-959X Roč. 7, č. 4 (2016), s. 604-613
Poč.str.	10 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	DE - Německo
Klíč. slova	albumin digestion ; tick ; proteolysis ; gut
Vědní obor RIV	ED - Fyziologie
Vědní obor RIV – spolupráce	Ústav organické chemie a biochemie - Biochemie
CEP	GA13-11043S GA ČR - Grantová agentura ČR
	GA14-33693S GA ČR - Grantová agentura ČR
	LO1302 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	BC-A - RVO:60077344 ; UOCHB-X - RVO:61388963
UT WOS	000379279000008
EID SCOPUS	84951806339
DOI	10.1016/j.ttbdis.2015.12.014
Anotace	Host blood proteins, represented mainly by hemoglobin and serum albumin, serve as the ultimate source of amino acids needed for de novo protein synthesis during tick development and reproduction. While uptake and processing of hemoglobin by tick gut cells have been studied in detail, molecular mechanisms of host serum albumin degradation remain unknown. In this work, we have used artificial membrane feeding of Ixodes ricinus females on a hemoglobin-free diet in order to characterize the proteolytic machinery involved in albuminolysis. Morphological comparisons of ticks fed on whole blood (BF) and serum (SF) at microscopic and ultrastructural levels showed that albumin and hemoglobin have different trafficking routes in tick gut cells. Analysis in vitro with selective inhibitors demonstrated that albumin is degraded at an acidic pH by a network of cysteine and aspartic peptidases with predominant involvement of cysteine cathepsins having endo- and exopeptidase activities. The cleavage map of albumin and the roles of individual peptidases in albumin degradation were determined. These results indicate that the albuminolytic pathway is controlled by the same proteolytic system that is responsible for hemoglobinolysis. This was further supported by the overall similarity of gut peptidase profiles in SF and BF ticks at the transcriptional and enzymatic activity levels. In conclusion, our work provides evidence that although hemoglobin and albumin are transported differentially during heterophagy they are digested by a common multienzyme proteolytic network. This central digestive system, critical for successful blood feeding in tick females, thus represents a valuable target for novel anti-tick interventions.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2017

Počet záznamů: 1