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Cryptosporidium avium n. sp (Apicomplexa: Cryptosporidiidae) in birds
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SYSNO ASEP 0461918 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cryptosporidium avium n. sp (Apicomplexa: Cryptosporidiidae) in birds Tvůrce(i) Holubová, Nikola (BC-A) SAI, ORCID
Sak, Bohumil (BC-A) RID, ORCID
Horčičková, Michaela (BC-A) ORCID
Hlásková, Lenka (BC-A) SAI
Květoňová, Dana (BC-A) SAI
Menchaca, S. (US)
McEvoy, J. (US)
Kváč, Martin (BC-A) RID, RID, ORCIDZdroj.dok. Parasitology Research. - : Springer - ISSN 0932-0113
Roč. 115, č. 6 (2016), s. 2243-2251Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Cryptosporidium avium ; morphology ; molecular analyses ; transmission studies ; Cryptosporidium avian genotype V Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína CEP GA15-01090S GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000376101000013 EID SCOPUS 84959105567 DOI 10.1007/s00436-016-4967-8 Anotace The morphological, biological, and molecular characteristics of Cryptosporidium avian genotype V are described, and the species name Cryptosporidium avium is proposed to reflect its specificity for birds under natural and experimental conditions. Oocysts of C. avium measured 5.30-6.90 mu m (mean = 6.26 mu m) x 4.30-5.50 mu m (mean = 4.86 mu m) with a length to width ratio of 1.29 (1.14-1.47). Oocysts of C. avium obtained from four naturally infected red-crowned parakeets (Cyanoramphus novaezealandiae) were infectious for 6-month-old budgerigars (Melopsittacus undulatus) and hens (Gallus gallus f. domestica). The prepatent periods in both susceptible bird species was 11 days postinfection (DPI). The infection intensity of C. avium in budgerigars and hens was low, with a maximum intensity of 5000 oocysts per gram of feces. Oocysts of C. avium were microscopically detected at only 12-16 DPI in hens and 12 DPI in budgerigars, while PCR analyses revealed the presence of specific DNA in fecal samples from 11 to 30 DPI (the conclusion of the experiment). Cryptosporidium avium was not infectious for 8-week-old SCID and BALB/c mice (Mus musculus). Naturally or experimentally infected birds showed no clinical signs of cryptosporidiosis, and no pathology was detected. Developmental stages of C. avium were detected in the ileum and cecum using scanning electron microscopy. Phylogenetic analyses based on small subunit rRNA, actin, and heat shock protein 70 gene sequences revealed that C. avium is genetically distinct from previously described Cryptosporidium species. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2017
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