Počet záznamů: 1  

Species- and Strain-Specific Adaptation of the HSP70 Super Family in Pathogenic Trypanosomatids

  1. 1.
    SYSNO ASEP0461882
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevSpecies- and Strain-Specific Adaptation of the HSP70 Super Family in Pathogenic Trypanosomatids
    Tvůrce(i) Drini, S. (FR)
    Criscuolo, A. (FR)
    Lechat, P. (FR)
    Imamura, H. (BE)
    Skalický, Tomáš (BC-A)
    Rachidi, N. (FR)
    Lukeš, Julius (BC-A) RID, ORCID
    Dujardin, J.-C. (BE)
    Späth, G.F. (FR)
    Zdroj.dok.Genome Biology and Evolution. - : Oxford University Press - ISSN 1759-6653
    Roč. 8, č. 6 (2016), s. 1980-1995
    Poč.str.16 s.
    Forma vydáníTištěná - P
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovaLeishmania ; heat shock protein ; HSP70 ; evolution ; phylogeny ; synteny ; copy number variation ; gene lossgene loss
    Vědní obor RIVEB - Genetika a molekulární biologie
    CEPGA14-23986S GA ČR - Grantová agentura ČR
    Institucionální podporaBC-A - RVO:60077344
    UT WOS000386368200010
    EID SCOPUS85000524338
    DOI10.1093/gbe/evw140
    AnotaceAll eukaryotic genomes encode multiple members of the heat shock protein 70 (HSP70) family, which evolved distinctive structural and functional features in response to specific environmental constraints. Phylogenetic analysis of this protein family thus can inform on genetic and molecular mechanisms that drive species-specific environmental adaptation. Here we use the eukaryotic pathogen Leishmania spp. as a model system to investigate the evolution of the HSP70 protein family in an early-branching eukaryote that is prone to gene amplification and adapts to cytotoxic host environments by stress-induced and chaperone-dependent stage differentiation. Combining phylogenetic and comparative analyses of trypanosomatid genomes, draft genome of Paratrypanosoma and recently published genome sequences of 204 L. donovani field isolates, we gained unique insight into the evolutionary dynamics of the Leishmania HSP70 protein family. We provide evidence for (i) significant evolutionary expansion of this protein family in Leishmania through gene amplification and functional specialization of highly conserved canonical HSP70 members, (ii) evolution of trypanosomatid-specific, non-canonical family members that likely gained ATPase-independent functions, and (iii) loss of one atypical HSP70 member in the Trypanosoma genus. Finally, we reveal considerable copy number variation of canonical cytoplasmic HSP70 in highly related L. donovani field isolates, thus identifying this locus as a potential hot spot of environment-genotype interaction. Our data draw a complex picture of the genetic history of HSP70 in trypanosomatids that is driven by the remarkable plasticity of the Leishmania genome to undergo massive intra-chromosomal gene amplification to compensate for the absence of regulated transcriptional control in these parasites
    PracovištěBiologické centrum (od r. 2006)
    KontaktDana Hypšová, eje@eje.cz, Tel.: 387 775 214
    Rok sběru2017
Počet záznamů: 1  

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