Počet záznamů: 1  

High-throughput fluorescence screening assay for the identification and comparison of antimicrobial peptides’ activity on various yeast species

  1. 1.
    SYSNO ASEP0461653
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevHigh-throughput fluorescence screening assay for the identification and comparison of antimicrobial peptides’ activity on various yeast species
    Tvůrce(i) Kodedová, Marie (FGU-C) RID, ORCID
    Sychrová, Hana (FGU-C) RID, ORCID
    Zdroj.dok.Journal of Biotechnology. - : Elsevier - ISSN 0168-1656
    Roč. 233, Sep 10 (2016), s. 26-33
    Poč.str.8 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovadiS-C3(3) assay ; antimicrobial peptide ; Candida ; membrane potential ; microplate reader
    Vědní obor RIVEE - Mikrobiologie, virologie
    CEPTA04010638 GA TA ČR - Technologická agentura ČR
    ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000380819300004
    EID SCOPUS84978253165
    DOI10.1016/j.jbiotec.2016.06.023
    AnotaceNew antifungal compounds that circumvent the resistance of the pathogen by directly damaging yeast cell surface structures are promising agents for the treatment of fungal infections, due to their different mechanism of action from current clinically used antifungal drugs. We present here a rapid and cost-effective fluorescence method suitable for identifying new potent drugs that directly target yeast cell surface structures, causing cell permeabilization and thus bypassing the multidrug resistance mechanisms of pathogens. The fluorescence assay enabled us to detect with high sensitivity damage to the Candida plasma membrane (its hyperpolarization and permeabilization) as a result of short-term exposure to the antifungal compounds. Results can be obtained in 1–2 h with minimal effort and consumption of the tested compounds, also 96 samples can be analysed simultaneously. We used this method to study antimicrobial peptides isolated from the venom of bees and their synthetic analogs, compare the potency of the peptides and determine their minimal effective concentrations. The antimicrobial peptides were able to kill yeast cells at low concentrations within a 15-min treatment, the LL-III peptide exhibited a broad spectrum of antifungal activity on various Saccharomyces, pathogenic Candida and osmotolerant yeast species.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2017
Počet záznamů: 1  

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