Počet záznamů: 1

PLK1 regulates spindle formation kinetics and APC/C activation in mouse zygote

SYSNO ASEP	0460308
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	PLK1 regulates spindle formation kinetics and APC/C activation in mouse zygote
Tvůrce(i)	Baran, V. (SK) Brzáková, Adéla (UZFG-Y) Rehák, P. (SK) Kovaříková, V. (CZ) Šolc, Petr (UZFG-Y)_{RID, ORCID}
Zdroj.dok.	Zygote. - : Cambridge University Press - ISSN 0967-1994 Roč. 24, č. 3 (2016), s. 338-345
Poč.str.	8 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	APC/C ; BI2536 ; live cell imaging ; mouse zygote ; PLK1 ; securin ; spindle assembly
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	ED2.1.00/03.0124 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	LH12057 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	UZFG-Y - RVO:67985904
UT WOS	000376277900003
EID SCOPUS	84968813929
DOI	10.1017/S0967199415000246
Anotace	Polo-like kinase 1 (PLK1) is involved in essential events of cell cycle including mitosis in which it participates in centrosomal microtubule nucleation, spindle bipolarity establishment and cytokinesis. Although PLK1 function has been studied in cycling cancer cells, only limited data are known about its role in the first mitosis of mammalian zygotes. During the 1-cell stage of mouse embryo development, the acentriolar spindle is formed and the shift from acentriolar to centrosomal spindle formation progresses gradually throughout the preimplantation stage, thus providing a unique possibility to study acentriolar spindle formation. We have shown previously that PLK1 activity is not essential for entry into first mitosis, but is required for correct spindle formation and anaphase onset in 1-cell mouse embryos. In the present study, we extend this knowledge by employing quantitative confocal live cell imaging to determine spindle formation kinetics in the absence of PLK1 activity and answer the question whether metaphase arrest at PLK1-inhibited embryos is associated with low anaphase-promoting complex/cyclosome (APC/C) activity and consequently high securin level. We have shown that inhibition of PLK1 activity induces a delay in onset of acentriolar spindle formation during first mitosis. Although these PLK1-inhibited 1-cell embryos were finally able to form a bipolar spindle, not all chromosomes were aligned at the metaphase equator. PLK1-inhibited embryos were arrested in metaphase without any sign of APC/C activation with high securin levels. Our results document that PLK1 controls the onset of spindle assembly and spindle formation, and is essential for APC/C activation before anaphase onset in mouse zygotes.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2017

Počet záznamů: 1