Počet záznamů: 1  

Transcriptional profiling of dividing tumor cells detects intratumor heterogeneity linked to cell proliferation in a brain tumor model

  1. 1.
    SYSNO ASEP0459262
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevTranscriptional profiling of dividing tumor cells detects intratumor heterogeneity linked to cell proliferation in a brain tumor model
    Tvůrce(i) Endaya, B. (AU)
    Lam, P.Y.P. (SG)
    Meedeniya, A.C.B. (AU)
    Neužil, Jiří (BTO-N) RID
    Zdroj.dok.Molecular Oncology. - : Wiley - ISSN 1574-7891
    Roč. 10, č. 1 (2016), s. 126-137
    Poč.str.12 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovaIntratumor heterogeneity ; Click chemistry ; Proliferation ; Gene profiling
    Vědní obor RIVFD - Onkologie a hematologie
    CEPED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaBTO-N - RVO:86652036
    UT WOS000369560300009
    DOI10.1016/j.molonc.2015.09.001
    AnotaceIntratumor heterogeneity is a primary feature of high-grade gliomas, complicating their therapy. As accumulating evidence suggests that intratumor heterogeneity is a consequence of cellular subsets with different cycling frequencies, we developed a method for transcriptional profiling of gliomas, using a novel technique to dissect the tumors into two fundamental cellular subsets, namely, the proliferating and non-proliferating cell fractions. The tumor fractions were sorted whilst maintaining their molecular integrity, by incorporating the thymidine analog 5-ethynyl-2'-deoxyuridine into actively dividing cells. We sorted the actively dividing versus non-dividing cells from cultured glioma cells, and parental and clonally derived orthotopic tumors, and analyzed them for a number of transcripts. While there was no significant difference in the transcriptional profiles between the two cellular subsets in cultured glioma cells, we demonstrate similar to 2-6 fold increase in transcripts of cancer and neuronal stem cell and tumor cell migration/invasion markers, and similar to 2-fold decrease in transcripts of markers of hypoxia and their target genes, in the dividing tumor cells of the orthotopic glioma when compared to their non-proliferative counterparts. This suggests the influence of the brain microenvironment in transcriptional regulation and, thereby, the physiology of glioma cells in vivo. When clonal glioma cells were derived from a parental glioma and the resultant orthotopic tumors were compared, their transcriptional profiles were closely correlated to tumor aggression and consequently, survival of the experimental animals. This study demonstrates the resolution of intratumor heterogeneity for profiling studies based on cell proliferation, a defining feature of cancers, with implications for treatment design.
    PracovištěBiotechnologický ústav
    KontaktMonika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
    Rok sběru2017
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.