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DNA damage response during mouse oocyte maturation

SYSNO ASEP	0458917
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	DNA damage response during mouse oocyte maturation
Tvůrce(i)	Mayer, Alexandra (UZFG-Y)_RID Baran, Vladimír (UZFG-Y) Sakakibara, Y. (JP) Brzáková, Adéla (UZFG-Y) Ferencová, Ivana (UZFG-Y)_ORCID Motlík, Jan (UZFG-Y)_{RID, ORCID} Kitajima, T. (JP) Schultz, R. M. (US) Šolc, Petr (UZFG-Y)_{RID, ORCID}
Zdroj.dok.	Cell Cycle. - : Taylor & Francis - ISSN 1538-4101 Roč. 15, č. 4 (2016), s. 546-558
Poč.str.	13 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	double strand DNA breaks ; DNA damage ; MRE11 ; meiotic maturation ; mouse oocytes
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	LH12057 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	ED2.1.00/03.0124 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	UZFG-Y - RVO:67985904
UT WOS	000372110000014
EID SCOPUS	84961285243
DOI	10.1080/15384101.2015.1128592
Anotace	Because low levels of DNA double strand breaks (DSBs) appear not to activate the ATM-mediated prophase I checkpoint in full-grown oocytes, there may exist mechanisms to protect chromosome integrity during meiotic maturation. Using live imaging we demonstrate that low levels of DSBs induced by the radiomimetic drug Neocarzinostatin (NCS) increase the incidence of chromosome fragments and lagging chromosomes but do not lead to APC/C activation and anaphase onset delay. The number of DSBs, represented by gamma H2AX foci, significantly decreases between prophase I and metaphase II in both control and NCS-treated oocytes. Transient treatment with NCS increases >2-fold the number of DSBs in prophase I oocytes, but less than 30% of these oocytes enter anaphase with segregation errors. MRE11, but not ATM, is essential to detect DSBs in prophase I and is involved in H2AX phosphorylation during metaphase I. Inhibiting MRE11 by mirin during meiotic maturation results in anaphase bridges and also increases the number of gamma H2AX foci in metaphase II. Compromised DNA integrity in mirin-treated oocytes indicates a role for MRE11 in chromosome integrity during meiotic maturation.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2017

Počet záznamů: 1