Počet záznamů: 1

The influence of erythrocyte maturity on ion transport and membrane lipid composition in the rat

SYSNO ASEP	0458522
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	The influence of erythrocyte maturity on ion transport and membrane lipid composition in the rat
Tvůrce(i)	Vokurková, Martina (FGU-C)_RID Rauchová, Hana (FGU-C)_{RID, ORCID} Dobešová, Zdenka (FGU-C) Loukotová, Jana (FGU-C) Nováková, O. (CZ) Kuneš, Jaroslav (FGU-C)_{RID, ORCID} Zicha, Josef (FGU-C)_{RID, ORCID, SAI}
Zdroj.dok.	Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408 Roč. 65, č. 1 (2016), s. 91-99
Poč.str.	9 s.
Jazyk dok.	eng - angličtina
Země vyd.	CZ - Česká republika
Klíč. slova	reticulocytes ; immature erythrocytes ; mean cellular hemoglobin content ; membrane phospholipids ; membrane cholesterol
Vědní obor RIV	ED - Fyziologie
CEP	NV15-25396A GA MZd - Ministerstvo zdravotnictví
	GAP304/12/0259 GA ČR - Grantová agentura ČR
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000377529800010
EID SCOPUS	84963830443
Anotace	Significant relationship between ion transport and membrane lipid composition (cholesterol, total phospholipids and sphingomyelins) were found in erythrocytes of salt hypertensive Dahl rats. In these animals mean cellular hemoglobin content correlated negatively with Na+-K+ pump activity and Na+ leak but positively with Na+-K+ cotransport activity. Immature erythrocytes exhibit lower mean cellular hemoglobin content (MCHC) than mature ones. The aim of the present study was to find a relationship between erythrocyte maturity, membrane lipid composition and ion transport activity in Wistar rats aged three months which were subjected to repeated hemorrhage (blood loss 2 ml/day for 6 days) to enrich circulating erythrocytes with immature forms. Immature and mature erythrocyte fractions in control and hemorrhaged rats were separated by repeated centrifugation. Hemorrhaged rats had increased number of reticulocytes but reduced hematocrit and MCHC compared to control rats. Immature erythrocytes of hemorrhaged rats differed from mature ones of control animals by elevated Na+-K+ pump activity, reduced Na+-K+ cotransport activity and increased Rb+ leak. These ion transport changes in immature erythrocytes were accompanied by higher concentration of total phospholipids in their cell membranes. Membrane phospholipid content correlated positively with Na+-K+ pump activity and cation leaks but negatively with Na+-K+ cotransport activity. Moreover, they were also negatively related with MCHC which correlated negatively with Na+-K+ pump activity and Rb+ leak but positively with Na+- K+ cotransport activity. Thus certain abnormalities of erythrocyte ion transport and membrane lipid composition detected in hypertensive animals might be caused by higher incidence of immature cells.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2017

Počet záznamů: 1