Počet záznamů: 1
Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens
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SYSNO ASEP 0453455 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens Tvůrce(i) Synytsya, A. (CZ)
Choi, D. J. (KR)
Pohl, Radek (UOCHB-X) RID, ORCID
Na, Y. S. (KR)
Capek, P. (SK)
Lattová, E. (SK)
Taubner, T. (CZ)
Choi, J. W. (KR)
Lee, C. W. (KR)
Park, J. K. (KR)
Kim, W. J. (KR)
Kim, S. M. (KR)
Lee, J. (KR)
Park, Y. I. (KR)Celkový počet autorů 14 Zdroj.dok. Marine Biotechnology - ISSN 1436-2228
Roč. 17, č. 6 (2015), s. 718-735Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova alga Maesaengi (Capsosiphon fulvescens) ; ulvan ; monosaccharide composition ; structure ; anti-coagulant activity Vědní obor RIV EI - Biotechnologie a bionika Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000364512500003 EID SCOPUS 84946495405 DOI 10.1007/s10126-015-9643-y Anotace Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010). However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount. These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked l-rhamnose-3-sulphate and d-xylose residues (ulvobiose U-3s) carrying monomeric d-glucuronic acid or d-glucuronic acid-3-sulphate on O-2 of some l-rhamnose-3-sulphate units as the side chains. The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2016
Počet záznamů: 1