Počet záznamů: 1
Complete mitochondrial genomes and nuclear ribosomal RNA operons of two species of Diplostomum (Platyhelminthes: Trematoda): a molecular resource for taxonomy and molecular epidemiology of important fish pathogens
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SYSNO ASEP 0447676 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Complete mitochondrial genomes and nuclear ribosomal RNA operons of two species of Diplostomum (Platyhelminthes: Trematoda): a molecular resource for taxonomy and molecular epidemiology of important fish pathogens Tvůrce(i) Brabec, Jan (BC-A) RID, ORCID
Kostadinova, Aneta (BC-A) RID
Scholz, Tomáš (BC-A) RID, ORCID
Littlewood, D. T. J. (GB)Zdroj.dok. Parasites & Vectors. - : BioMed Central - ISSN 1756-3305
Roč. 8, JUN 19 2015 (2015), s. 336Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Diplostomum (Platyhelminthes: Trematoda) ; fish pathogens ; mitochondrial genome ; ribosomal RNA ; illumina next-generation sequencing ; phylogeny Vědní obor RIV EG - Zoologie CEP EE2.3.30.0032 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA15-14198S GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000356668000001 DOI 10.1186/s13071-015-0949-4 Anotace Results: We characterised novel complete mt genomes and nuclear rRNA operons of two closely related species, Diplostomum spathaceum and D. pseudospathaceum. Comparative mt genome assessment revealed that the cox1 gene and its 'barcode' region used for molecular identification are the most conserved regions; instead, nad4 and nad5 genes were identified as most promising molecular diagnostic markers. Using the novel data, we provide the first genome wide estimation of the phylogenetic relationships of the order Diplostomida, one of the two fundamental lineages of the Digenea. Analyses of the mitogenomic data invariably recovered the Diplostomidae as a sister lineage of the order Plagiorchiida rather than as a basal lineage of the Diplostomida as inferred in rDNA phylogenies; this was concordant with the mt gene order of Diplostomum spp. exhibiting closer match to the conserved gene order of the Plagiorchiida. Conclusions: Complete sequences of the mt genome and rRNA operon of two species of Diplostomum provide a valuable resource for novel genetic markers for species delineation and large-scale molecular epidemiology and disease ecology studies based on the most accessible life-cycle stages of eye flukes. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2016
Počet záznamů: 1