Počet záznamů: 1

Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig

SYSNO ASEP	0443826
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig
Tvůrce(i)	Plánská, Daniela (UZFG-Y) Burocziová, Monika (MBU-M) Strnádel, Ján (UZFG-Y)_RID Horák, Vratislav (UZFG-Y)_{RID, ORCID}
Zdroj.dok.	Acta histochemica et cytochemica - ISSN 0044-5991 Roč. 48, č. 1 (2015), s. 15-26
Poč.str.	12 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	DE - Německo
Klíč. slova	collagen IV ; laminin ; MeLiM ; porcine melanoma ; spontaneous regression
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	ED2.1.00/03.0124 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	UZFG-Y - RVO:67985904 ; MBU-M - RVO:61388971
UT WOS	000354928000003
EID SCOPUS	84923648899
DOI	10.1267/ahc.14020
Anotace	Spontaneous regression (SR) of human melanoma is a rare, well-documented phenomenon that is not still fully understood. Its detailed study cannot be performed in patients due to ethical reasons. Using the Melanoma-bearing Libechov Minipig (MeLiM) animals of various ages (from 3 weeks to 8 months) we implemented a long-term monitoring of melanoma growth and SR. We focused on immunohistochemical detection of two important extracellular matrix proteins, collagen IV and laminin, which are associated with cancer. We showed that SR of melanoma is a highly dynamic process. The expression of collagen IV and laminin correlated with changes in population of melanoma cells. Tumours of 3-week-old animals consisted primarily of melanoma cells with a granular expression of collagen IV and laminin around them. Thereafter, melanoma cells were gradually destroyed and tumour tissue was rebuilt into the connective tissue. Collagen IV expression slightly increased in tumours of 10-week-old pigs showing extracellular fibrous appearance. In tumours of older animals, areas lacking melanoma cells demonstrated a low expression and areas still containing melanoma cells a high expression of both proteins. We considered the age of 10 weeks as a turning point in the transition between tumour growth and SR of the MeLiM melanoma.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2016

Počet záznamů: 1