Počet záznamů: 1
Detection of Leishmania donovani and L. tropica in ethiopian wild rodents
- 1.
SYSNO ASEP 0441939 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Detection of Leishmania donovani and L. tropica in ethiopian wild rodents Tvůrce(i) Kassahun, A. (CZ)
Sádlová, J. (CZ)
Dvořák, V. (CZ)
Košťálová, T. (CZ)
Rohoušová, I. (CZ)
Frynta, D. (CZ)
Aghová, Tatiana (UBO-W) ORCID, SAI
Yasur-Landau, D. (IL)
Lemma, W. (ET)
Hailu, A. (ET)
Baneth, G. (IL)
Warburg, A. (IL)
Volf, P. (CZ)
Votýpka, J. (CZ)Celkový počet autorů 14 Zdroj.dok. Acta Tropica. - : Elsevier - ISSN 0001-706X
Roč. 145, May 2015 (2015), s. 39-44Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Leishmania donovani ; Leishmania tropica ; Phlebotomine sand fly ; Rodents ; kDNA ; ITS1 Vědní obor RIV EG - Zoologie CEP GAP506/10/0983 GA ČR - Grantová agentura ČR Institucionální podpora UBO-W - RVO:68081766 UT WOS 000353007000007 EID SCOPUS 84924037319 DOI 10.1016/j.actatropica.2015.02.006 Anotace Human visceral (VL) and cutaneous (CL) leishmaniasis are important infectious diseases affecting countries in East Africa that remain endemic in several regions of Ethiopia. The transmission and epidemiology of the disease is complicated due to the complex life cycle of the parasites and the involvement of various Leishmania spp., sand fly vectors, and reservoir animals besides human hosts. Particularly in East Africa, the role of animals as reservoirs for human VL remains unclear. Isolation of Leishmania donovani parasites from naturally infected rodents has been reported in several endemic countries; however, the status of rodents as reservoirs in Ethiopia remains unclear. Here, we demonstrated natural Leishmania infections in rodents. Animals were trapped in 41 localities of endemic and non-endemic areas in eight geographical regions of Ethiopia and DNA was isolated from spleens of 586 rodents belonging to 21 genera and 38 species. Leishmania infection was evaluated by real-time PCR of kinetoplast (k)DNA and confirmed by sequencing of the PCR products. Subsequently, parasite species identification was confirmed by PCR and DNA sequencing of the 18S ribosomal RNA internal transcribed spacer one (ITS1) gene. Out of fifty (8.2%) rodent specimens positive for Leishmania kDNA-PCR and sequencing, 10 were subsequently identified by sequencing of the ITS1 showing that five belonged to the L. donovani complex and five to L. tropica. Forty nine kDNA-positive rodents were found in the endemic localities of southern and eastern Ethiopia while only one was identified from northwestern Ethiopia. Moreover, all the ten ITS1-positive rodents were captured in areas where human leishmaniasis cases have been reported and potential sand fly vectors occur. Our findings suggest the eco-epidemiological importance of rodents in these foci of leishmaniasis and indicate that rodents are likely to play a role in the transmission of leishmaniasis in Ethiopia, possibly as reservoir hosts. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2016
Počet záznamů: 1