Počet záznamů: 1
Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve
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SYSNO ASEP 0440580 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve Tvůrce(i) Dubový, P. (CZ)
Brázda, Václav (BFU-R) RID, ORCID
Klusáková, I. (CZ)
Hradilová-Svíženská, I. (CZ)Celkový počet autorů 4 Zdroj.dok. Journal of Neuroinflammation. - : Filtch Solutions
Roč. 10, č. 55 (2013)Poč.str. 22 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Unilateral nerve injury ; Contralateral reaction ; Remote ganglia Vědní obor RIV BO - Biofyzika CEP ED1.1.00/02.0068 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BFU-R - RVO:68081707 UT WOS 000319195400001 DOI 10.1186/1742-2094-10-55 Anotace Background: Current research implicates interleukin (IL)-6 as a key component of the nervous-system response to injury with various effects. Methods: We used unilateral chronic constriction injury (CCI) of rat sciatic nerve as a model for neuropathic pain. Immunofluorescence, ELISA, western blotting and in situ hybridization were used to investigate bilateral changes in IL-6 protein and mRNA in both lumbar (L4-L5) and cervical (C7-C8) dorsal root ganglia (DRG) following CCI. The operated (CCI) and sham-operated (sham) rats were assessed after 1, 3, 7, and 14 days. Withdrawal thresholds for mechanical hyperalgesia and latencies for thermal hyperalgesia were measured in both ipsilateral and contralateral hind and fore paws.Results: The ipsilateral hind paws of all CCI rats displayed a decreased threshold of mechanical hyperalgesia and withdrawal latency of thermal hyperalgesia, while the contralateral hind and fore paws of both sides exhibited no significant changes in mechanical or thermal sensitivity. No significant behavioral changes were found in the hind and fore paws on either side of the sham rats, except for thermal hypersensitivity, which was present bilaterally at 3 days. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2015
Počet záznamů: 1