Počet záznamů: 1
Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria
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SYSNO ASEP 0439950 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria Tvůrce(i) Hnilicová, Jarmila (MBU-M) RID, ORCID
Jirát-Matějčková, Jitka (MBU-M)
Šiková, Michaela (MBU-M) ORCID
Pospíšil, Jiří (MBU-M) ORCID, RID
Halada, Petr (MBU-M) RID, ORCID
Pánek, Josef (MBU-M) RID
Krásný, Libor (MBU-M) RID, ORCIDCelkový počet autorů 7 Zdroj.dok. Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 42, č. 18 (2014), s. 11763-11776Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova ESCHERICHIA-COLI ; 6S RNA ; NONCODING RNA Vědní obor RIV EE - Mikrobiologie, virologie CEP GBP305/12/G034 GA ČR - Grantová agentura ČR GP13-27150P GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000347687100047 Anotace Small RNAs (sRNAs) are molecules essential for a number of regulatory processes in the bacterial cell. Here we characterize Ms1, a sRNA that is highly expressed in Mycobacterium smegmatis during stationary phase of growth. By glycerol gradient ultracentrifugation, RNA binding assay, and RNA co-immunoprecipitation, we show that Ms1 interacts with the RNA polymerase (RNAP) core that is free of the primary sigma factor (sigma(A)) or any other sigma factor. This contrasts with the situation in most other species where it is 6S RNA that interacts with RNAP and this interaction requires the presence of sigma(A). The difference in the interaction of the two types of sR-NAs (Ms1 or 6S RNA) with RNAP possibly reflects the difference in the composition of the transcriptional machinery between mycobacteria and other species. Unlike Escherichia coli, stationary phase M. smegmatis cells contain relatively few RNAP molecules in complex with sigma(A). Thus, Ms1 represents a novel type of small RNAs interacting with RNAP. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2015
Počet záznamů: 1