Počet záznamů: 1
CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation
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SYSNO ASEP 0439939 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation Tvůrce(i) Dušková, E. (CZ)
Hnilicová, Jarmila (MBU-M) RID, ORCID
Staněk, D. (CZ)Celkový počet autorů 3 Zdroj.dok. RNA biology. - : Taylor & Francis - ISSN 1547-6286
Roč. 11, č. 7 (2014), s. 1-10Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova alternative splicing ; fibronectin ; p300 Vědní obor RIV EE - Mikrobiologie, virologie CEP GBP305/12/G034 GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000342901600013 DOI 10.4161/rna.29441 Anotace Histone acetylation modulates alternative splicing of several hundred genes. Here, we tested the role of the histone acetyltransferase p300 in alternative splicing and showed that knockdown of p300 promotes inclusion of the fibronectin (FN1) alternative EDB exon. p300 associates with CRE sites in the promoter via the CREB transcription factor. We created mini-gene reporters driven by an artificial promoter containing CRE sites. Both deletion and mutation of the CRE site affected EDB alternative splicing in the same manner as p300 knockdown. Next we showed that p300 controls histone H4 acetylation along the FN1 gene. Consistently, p300 depletion and CRE deletion/mutation both reduced histone H4 acetylation on mini-gene reporters. Finally, we provide evidence that the effect of CRE inactivation on H4 acetylation and alternative splicing is counteracted by the inhibition of histone deacetylases. Together, these data suggest that histone acetylation could be one of the mechanisms how promoter and promoter binding proteins influence alternative splicing Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2015
Počet záznamů: 1