Počet záznamů: 1
Potential of correlative luminescence and electron microscopy for detection and location of individual molecules in cells
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SYSNO ASEP 0438715 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Potential of correlative luminescence and electron microscopy for detection and location of individual molecules in cells Tvůrce(i) Klepárník, Karel (UIACH-O) RID, ORCID Celkový počet autorů 1 Zdroj.dok. 20th International workshop on "Single Molecule Spectroscopy and Ultrasensitive Analysis in the Life Sciences". Program and Abstract Book.. - : PiCoQuant GmbH, 2014
S. 128-128Poč.str. 1 s. Forma vydání Tištěná - P Akce International workshop on "Single Molecule Spectroscopy and Ultrasensitive Analysis in the Life Sciences" /20./ Datum konání 02.09.2015-05.09.2015 Místo konání Berlin-Adlershof Země DE - Německo Typ akce WRD Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova electron microscopy ; individual molecules ; cell Vědní obor RIV CB - Analytická chemie, separace CEP GA14-28254S GA ČR - Grantová agentura ČR Institucionální podpora UIACH-O - RVO:68081715 Anotace Rapidly growing field of correlative light and electron microscopy (CLEM) overcomes some drawbacks of EM together with a time consuming process of the localization of studied structures. This technique bridges the functionality of LM with the high resolution of EM. The dynamic data from an optical source are correlated with ultrastructural analysis done by EM by rendering an overlay image of the same area of the specimen. In the last few years, CLEM has become a unique tool for the investigation of relations between a structure and its biological function. The possibility to reliably map locations of receptors (or any protein) onto a cellular surface with a nanometer accuracy has initiated a tremendous interest in this technique. Usually, a specific antibody is conjugated with a high electron density luminophore nanoparticle to form a probe for tracing cellular objects. Under LM, the luminescence image shows the regions of interest and/or functional changes. Subsequently, an electron beam reveals examined structures highlighted by the nanoparticle dots with the nanometer resolution. A unique approach takes advantage of cathodoluminescence of quantum dots (QD). Electron beam excites QD similarly as a laser and the luminescence emission is detected using a sensitive light detector in the same device. Our investigation is focused on two transmembrane proteins, Fas receptor and Fas ligand. These membrane receptors switch the external apoptotic pathways typical for surveillance strategies of the immune system. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2015
Počet záznamů: 1