Počet záznamů: 1
Electrophoretic preconcentration and separation of cationic labeled saccharides
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SYSNO ASEP 0437860 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Electrophoretic preconcentration and separation of cationic labeled saccharides Tvůrce(i) Partyka, Jan (UIACH-O)
Foret, František (UIACH-O) RID, ORCIDCelkový počet autorů 2 Zdroj.dok. ITP & LACE 2014. Book of Abstracts. - : Grupo VLS Print Solution, 2014 / Guzman N. A. ; Taveres M. F. M.
S. 97-97Poč.str. 1 s. Forma vydání Online - E Akce International Symposium on Electro- and Liquid Phase-Separation Techniques /21./ and Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology /21./ Datum konání 04.10.2014-08.10.2014 Místo konání Natal Země BR - Brazílie Typ akce WRD Jazyk dok. eng - angličtina Země vyd. BR - Brazílie Klíč. slova saccharide ; AETMA ; electrophoresis Vědní obor RIV CB - Analytická chemie, separace CEP GAP301/11/2055 GA ČR - Grantová agentura ČR Institucionální podpora UIACH-O - RVO:68081715 Anotace In continuation of this work we have been exploring small molecules with permanent positive charge as cationic tags. Oligosaccharides and glycans were labeled by reductive amination and the resulting derivatized saccharides were analyzed by capillary electrophoresis coupled to contactless conductivity detection and electrospray ionization mass spectrometry. The quaternary ammonium based tags assured migration of the oligosaccharide species as cations with electrophoretic mobility independent of the pH of the background electrolyte. For transient isotachophoretic preconcentration, the sample was complemented with ammonium ions and injected as a long zone occupying up to 70% of the separation capillary length. The labeled species, injected in the nanomolar concentrations, were focused into narrow zones by transient isotachophoresis followed by the separation in capillary electrophoretic mode. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2015
Počet záznamů: 1