Počet záznamů: 1
Biosynthesis of Colabomycin E, a New Manumycin-Family Metabolite, Involves an Unusual Chain-Length Factor
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SYSNO ASEP 0434468 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biosynthesis of Colabomycin E, a New Manumycin-Family Metabolite, Involves an Unusual Chain-Length Factor Tvůrce(i) Petříčková, Kateřina (MBU-M) RID
Pospíšil, Stanislav (MBU-M) RID
Kuzma, Marek (MBU-M) ORCID, RID
Tylová, Tereza (MBU-M)
Jágr, Michal (MBU-M)
Tomek, P. (CZ)
Chroňáková, Alica (BC-A) RID, ORCID
Brabcová, E. (CZ)
Anděra, Ladislav (UMG-J) RID
Krištůfek, Václav (BC-A) RID
Petříček, Miroslav (MBU-M) RIDZdroj.dok. Chembiochem. - : Wiley - ISSN 1439-4227
Roč. 15, č. 9 (2014), s. 1334-1345Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova biosynthesis ; chain-length factors ; manumycins Vědní obor RIV CE - Biochemie CEP NT13012 GA MZd - Ministerstvo zdravotnictví Institucionální podpora MBU-M - RVO:61388971 ; BC-A - RVO:60077344 ; UMG-J - RVO:68378050 UT WOS 000337638400016 DOI 10.1002/cbic.201400068 Anotace Colabomycin E is a new member of the manumycin-type metabolites produced by the strain Streptomyces aureus SOK1/5-04 and identified by genetic screening from a library of streptomycete strains. The structures of colabomycin E and accompanying congeners were resolved. The entire biosynthetic gene cluster was cloned and expressed in Streptomyces lividans. Bioinformatic analysis and mutagenic studies identified components of the biosynthetic pathway that are involved in the formation of both polyketide chains. Recombinant polyketide synthases (PKSs) assembled from the components of colabomycin E and asukamycin biosynthetic routes catalyzing the biosynthesis of "lower" carbon chains were constructed and expressed in S. aureus SOK1/5-04 Delta colC11-14 deletion mutant. Analysis of the metabolites produced by recombinant strains provided evidence that in both biosynthetic pathways the length of the lower carbon chain is controlled by an unusual chain-length factor supporting biosynthesis either of a triketide in asukamycin or of a tetraketide in colabomycin E. Biological activity assays indicated that colabomycin E significantly inhibited IL-1 beta release from THP-1 cells and might thus potentially act as an anti-inflammatory agent Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2015
Počet záznamů: 1