Počet záznamů: 1  

Phospholipid lateral diffusion in phosphatidylcholine-sphingomyelin-cholesterol monolayers; Effects of oxidatively truncated phosphatidylcholines

  1. 1.
    SYSNO ASEP0434363
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevPhospholipid lateral diffusion in phosphatidylcholine-sphingomyelin-cholesterol monolayers; Effects of oxidatively truncated phosphatidylcholines
    Tvůrce(i) Parkkila, P. (FI)
    Štefl, Martin (UFCH-W)
    Olžyńska, Agnieszka (UFCH-W) RID
    Hof, Martin (UFCH-W) RID, ORCID
    Kinnunen, P. K. J. (FI)
    Zdroj.dok.Biochimica Et Biophysica Acta-Biomembranes. - : Elsevier - ISSN 0005-2736
    Roč. 1848, č. 1 (2015), s. 167-173
    Poč.str.7 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovaOxidatively truncated phosphatidylcholines ; Lateral diffusion ; Fluorescence correlation spectroscopy
    Vědní obor RIVCF - Fyzikální chemie a teoretická chemie
    CEPGBP208/12/G016 GA ČR - Grantová agentura ČR
    Institucionální podporaUFCH-W - RVO:61388955
    UT WOS000347509200021
    EID SCOPUS84908517990
    DOI10.1016/j.bbamem.2014.10.025
    AnotaceOxidative stress is involved in a number of pathological conditions and the generated oxidatively modified lipids influence membrane properties and functions, including lipid–protein interactions and cellular signaling. Brewster angle microscopy demonstrated oxidatively truncated phosphatidylcholines to promote phase separation in monolayers of 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC), sphingomyelin (SM) and cholesterol (Chol). More specifically, 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC), was found to increase the miscibility transition pressure of the SM/Chol-phase. Lateral diffusion of lipids is influenced by a variety of membrane properties, thus making it a sensitive parameter to observe the coexistence of different lipid phases, for instance. The dependence on lipid lateral packing of the lateral diffusion of fluorophore-containing phospholipid analogs was investigated in Langmuir monolayers composed of POPC, SM, and Chol and additionally containing oxidatively truncated phosphatidylcholines, using fluorescence correlation spectroscopy (FCS). To our knowledge, these are the first FCS results on miscibility transition in ternary lipid monolayers, confirming previous results obtained using Brewster angle microscopy on such lipid monolayers. Wide-field fluorescence microscopy was additionally employed to verify the transition, i.e. the loss and reformation of SM/Chol domains.
    PracovištěÚstav fyzikální chemie J.Heyrovského
    KontaktMichaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
    Rok sběru2016
Počet záznamů: 1  

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