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Novel human multiple myeloma cell line UHKT-893
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SYSNO ASEP 0423264 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Novel human multiple myeloma cell line UHKT-893 Tvůrce(i) Uherková, L. (CZ)
Vančurová, I. (CZ)
Vyhlídalová, I. (CZ)
Pleschnerová, M. (CZ)
Špička, I. (CZ)
Mihalová, R. (CZ)
Březinová, J. (CZ)
Hodný, Zdeněk (UMG-J) RID
Čermáková, K. (CZ)
Polanská, V. (CZ)
Marinov, I. (CZ)
Jedelský, P.L. (CZ)
Kuželová, K. (CZ)
Stöckbauer, P. (CZ)Zdroj.dok. Leukemia Research. - : Elsevier - ISSN 0145-2126
Roč. 37, č. 3 (2013), s. 320-326Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova human myeloma cell line ; human multiple myeloma ; plasma cell ; IL-6 dependence ; immunoglobulin ; free light chain Vědní obor RIV EB - Genetika a molekulární biologie Institucionální podpora UMG-J - RVO:68378050 UT WOS 000314871500017 DOI 10.1016/j.leukres.2012.12.004 Anotace We established and characterized a new IL-6 dependent multiple myeloma (MM) cell line UHKT-893 from the bone marrow of a relapsed 57-year-old woman. Using nephelometry, cells with plasma cell phenotype and morphology were found to secrete IgG and free kappa (κ)-light chain of immunoglobulin. κ-Light chain was also recognized intracellularly by flow cytometry and by mass spectrometry. VH4-39 region of IgVH genes was rearranged and somatically hypermutated. Cytogenetic analysis of cells revealed new chromosome abnormalities in all breakpoints unique in both MM patients and cell lines – t(1;6), t(1;11), t(5;15), t(5;21), +der(11;15) and der(16). IL-6 independent subline UHKT-893a was established by adaptation to descending IL-6 concentration, while the original cell line keeps on maintaining its IL-6 dependency. The cell line provides a suitable material for cellular and molecular studies of tumor abnormalities, with potentially unique mutagenic features of myeloma disease. It may be utilized for human hybridoma construction and vaccine development. Both IL-6 dependent and independent cell clones represent an important model for studies of myeloma cell growth and resistance emerging during targeted therapy. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2014
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