Počet záznamů: 1
Study of Possible Mechanisms Involved in the Inhibitory Effects of Coumarin Derivatives on Neutrophil Activity
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SYSNO ASEP 0421993 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Study of Possible Mechanisms Involved in the Inhibitory Effects of Coumarin Derivatives on Neutrophil Activity Tvůrce(i) Drábiková, K. (SK)
Perečko, T. (SK)
Nosáľ, R. (SK)
Harmatha, Juraj (UOCHB-X) RID, ORCID
Šmidrkal, J. (CZ)
Jančinová, V. (SK)Celkový počet autorů 6 Zdroj.dok. Oxidative Medicine and Cellular Longevity. - : Hindawi - ISSN 1942-0900
Roč. 2013, October (2013), 136570/1-136570/10Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova protein-kinase-C ; phagocyte NADPH oxidase ; antioxidant activity Vědní obor RIV CC - Organická chemie Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000327636900001 EID SCOPUS 84890048506 DOI 10.1155/2013/136570 Anotace To specify the site of action of the synthetic coumarin derivatives 7-hydroxy-3-(4'-hydroxyphenyl) coumarin (HHC) and 7-hydroxy-3-(4'-hydroxyphenyl) dihydrocoumarin (HHDC), we evaluated their effects on extra-and intracellular reactive oxygen species (ROS) formation in phorbol-myristate-13-acetate (PMA) stimulated human neutrophils. We studied also the effects of HHC and HHDC on possible molecular mechanisms which participate in the activation of NADPH oxidase, that is, on PKC activity, on phosphorylation of some PKC isoforms (alpha, beta II, and delta), and on phosphorylation of the NADPH oxidase subunit p40(phox). Without affecting cytotoxicity, both coumarines tested were effective inhibitors/scavengers of ROS produced by neutrophils on extracellular level. HHC markedly diminished oxidant production and also, intracellularly, decreased PKC activity and partly phosphorylation of PKC alpha, beta II. On the other hand, we did not observe any effect of coumarin derivatives on phosphorylation of PKC delta and on phosphorylation of the NADPH oxidase subunit p40(phox), which were suggested to be involved in the PMA-dependent intracellular activation process. In agreement with our previous findings, we assume that the different molecular structures of HHC and HHDC with their different physicochemical and free radical scavenging characteristics are responsible for their diverse effects on the parameters tested. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2014 Elektronická adresa http://www.hindawi.com/journals/omcl/2013/136570/
Počet záznamů: 1