Počet záznamů: 1  

Panurgines, novel antimicrobial peptides from the venom of communal bee Panurgus calcaratus (Hymenoptera: Andrenidae)

  1. 1.
    SYSNO ASEP0394219
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevPanurgines, novel antimicrobial peptides from the venom of communal bee Panurgus calcaratus (Hymenoptera: Andrenidae)
    Tvůrce(i) Čujová, Sabína (UOCHB-X) RID
    Slaninová, Jiřina (UOCHB-X)
    Monincová, Lenka (UOCHB-X)
    Fučík, Vladimír (UOCHB-X) RID
    Bednárová, Lucie (UOCHB-X) RID, ORCID
    Štokrová, Jitka (UOCHB-X)
    Hovorka, Oldřich (UOCHB-X)
    Voburka, Zdeněk (UOCHB-X)
    Straka, J. (CZ)
    Čeřovský, Václav (UOCHB-X) RID, ORCID
    Celkový počet autorů10
    Zdroj.dok.Amino Acids. - : Springer - ISSN 0939-4451
    Roč. 45, č. 1 (2013), s. 143-157
    Poč.str.15 s.
    Jazyk dok.eng - angličtina
    Země vyd.AT - Rakousko
    Klíč. slovaantimicrobial peptides ; wild bee venom ; CD spectroscopy ; large unilamellar vesicles ; electron microscopy
    Vědní obor RIVCE - Biochemie
    CEPGA203/08/0536 GA ČR - Grantová agentura ČR
    Institucionální podporaUOCHB-X - RVO:61388963
    UT WOS000320372500011
    EID SCOPUS84879202579
    DOI10.1007/s00726-013-1482-4
    AnotaceThree novel antimicrobial peptides (AMPs), named panurgines (PNGs), were isolated from the venom of the wild bee Panurgus calcaratus. The dodecapeptide of the sequence LNWGAILKHIIK-NH2 (PNG-1) belongs to the category of alpha-helical amphipathic AMPs. The other two cyclic peptides containing 25 amino acid residues and two intramolecular disulfide bridges of the pattern Cys8-Cys23 and Cys11-Cys19 have almost identical sequence established as LDVKKIICVACKIXPNPACKKICPK-OH (X=K, PNG-K and X=R, PNG-R). All three peptides exhibited antimicrobial activity against Gram-positive bacteria and Gram-negative bacteria, antifungal activity, and low hemolytic activity against human erythrocytes. We prepared a series of PNG-1 analogs to study the effects of cationicity, amphipathicity, and hydrophobicity on the biological activity. Several of them exhibited improved antimicrobial potency, particularly those with increased net positive charge. The linear analogs of PNG-K and PNG-R having all Cys residues substituted by alpha-amino butyric acid were inactive, thus indicating the importance of disulfide bridges for the antimicrobial activity. However, the linear PNG-K with all four cysteine residues unpaired, exhibited antimicrobial activity. PNG-1 and its analogs induced a significant leakage of fluorescent dye entrapped in bacterial membrane-mimicking large unilamellar vesicles as well as in vesicles mimicking eukaryotic cell membrane. On the other hand, PNG-K and PNG-R exhibited dye-leakage activity only from vesicles mimicking bacterial cell membrane.
    PracovištěÚstav organické chemie a biochemie
    Kontaktasep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
    Rok sběru2014
Počet záznamů: 1  

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