Počet záznamů: 1

Nanoparticle-Based Immunocytochemistry Reveals Microarchitecture of the Cell Nucleus

SYSNO ASEP	0389005
Druh ASEP	C - Konferenční příspěvek (mezinárodní konf.)
Zařazení RIV	Záznam nebyl označen do RIV
Název	Nanoparticle-Based Immunocytochemistry Reveals Microarchitecture of the Cell Nucleus
Tvůrce(i)	Hozák, Pavel (UMG-J)_{RID, ORCID}
Zdroj.dok.	Beyond the Limit of Histochemistry - 14th INTERNATIONAL CONGRESS OF HISTOCHEMISTRY AND CYTOCHEMISTRY. - Kyoto : International Federation of Societies for Histochemistry and Cytochemistry, Japan Society of Histochemistry, 2012
Poč.str.	1 s.
Akce	14th International Congress of Histochemistry and Cytochemistry
Datum konání	26.08.2012-29.08.2012
Místo konání	Kyoto
Země	JP - Japonsko
Typ akce	WRD
Jazyk dok.	eng - angličtina
Země vyd.	JP - Japonsko
Klíč. slova	PIP2 ; NMI ; cell nucleus
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GAP305/11/2232 GA ČR - Grantová agentura ČR
	LC545 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	FRTI3588 GA MPO - Ministerstvo průmyslu a obchodu
Institucionální podpora	UMG-J - RVO:68378050
CEZ	AV0Z50520514 - UMG-J (2005-2011)
Anotace	I will summarize the current possibilities of TEM visualization of molecules in cells. In order to overcome the current limitations of immunodetection, we prepared a set of novel nanoparticles (NPs) which fulfill several criteria: size in the frame of 5-12 nm, small size distribution, good contrast and stability in the electron microscope, stability of colloidal solution during conjugation, and surface properties allowing for conjugation with antibodies With the use of novel NPs, various combinations with commercial gold NPs can be made to obtain a set for simultaneous labeling. For the first time in ultrastructural histochemistry, up to five molecular targets can be identified simultaneously. These methods allowed us to progress with understanding some novel molecular interactions in the cell nucleus. I will discuss interactions of phosphatidylinositol-4,5-bisphosphate (PIP2) and nuclear myosin I (NMI) which are involved in regulation of gene expression. PIP2 resides in the nucleus in a different form than the classical bilayer membrane, apparently forming specific nuclear protein complexes. Our data suggest that nucleolar PIP2 might serve as a transcription factor for ribosomal genes. We therefore investigated PIP distribution in cell nuclei with a special attention to nucleoli by ultrastructural tomography, and mapped PIP colocalization with various factors involved in RNA pol I transcription. We also showed in living cells that NM1 binds to PIP2 in the cell nucleus, and this was further confirmed by electron microscopy and molecular approaches. The results will be discussed in the frame of the current model of the nucleolus and lipid functions in the cell nucleus.
Pracoviště	Ústav molekulární genetiky
Kontakt	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Rok sběru	2014

Počet záznamů: 1