Počet záznamů: 1
Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1
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SYSNO ASEP 0381622 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1 Tvůrce(i) Hlaváčková, Veronika (UMG-J)
Zabel, U. (DE)
Franková, Daniela (UMG-J)
Batz, J. (DE)
Hoffmann, C. (DE)
Prezeau, L. (FR)
Pin, J. P. (FR)
Blahoš, Jaroslav (UMG-J) RID
Lohse, M. J. (DE)Zdroj.dok. Science Signaling. - : American Association for the Advancement of Science - ISSN 1945-0877
Roč. 5, č. 237 (2012), ra59Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova G-protein coupled receptor ; metabotropic glutamate receptor 1 ; class C GPCR Vědní obor RIV EB - Genetika a molekulární biologie CEP GA303/08/1591 GA ČR - Grantová agentura ČR LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GAP303/12/2408 GA ČR - Grantová agentura ČR CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000307885200002 DOI 10.1126/scisignal.2002720 Anotace The metabotropic glutamate receptor 1 (mGluR1), a class C member of the heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptor family, is a constitutive dimer that regulates excitatory neurotransmission. We investigated the role of homodimer formation in mGluR1 activation by examining activation-dependent inter-and intrasubunit conformational changes by fluorescence resonance energy transfer (FRET). We inserted yellow and cyan fluorescent proteins in the second intracellular loop and at the carboxyl terminus of mGluR1 to act as FRET sensors and expressed these proteins in human embryonic kidney 293 cells. Agonist-dependent activation of these mGluR1 chimeras rapidly increased the intersubunit FRET, suggesting rapid movement of the subunits relative to each other. After intersubunit movement, the intrasubunit FRET decreased, reflecting conformational changes within a subunit. Cotransfection of chimeric receptor subunits that were capable or incapable of G protein coupling revealed that only a single subunit assumes an active state in an mGluR1 receptor dimer. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2013
Počet záznamů: 1