Počet záznamů: 1
Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration
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SYSNO ASEP 0379290 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration Tvůrce(i) Hrstka, M. (CZ)
Urban, Otmar (UEK-B) RID, ORCID, SAI
Babák, L. (CZ)Celkový počet autorů 3 Zdroj.dok. Chemical Papers. - : Springer - ISSN 0366-6352
Roč. 66, č. 9 (2012), s. 836-841Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. SK - Slovensko Klíč. slova Rubisco content ; Rubisco activity ; seasonal changes ; elevated CO2 concentrations ; Fagus sylvatica ; Picea abies Vědní obor RIV EH - Ekologie - společenstva CEP IAA600870701 GA AV ČR - Akademie věd LM2010007 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0073 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z60870520 - UEK-B (2005-2011) UT WOS 000305563300006 DOI 10.2478/s11696-012-0188-5 Anotace We studied Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) content and activity in juvenile tree species - broadleaved Fagus sylvatica L. and coniferous Picea abies (L.) Karsten exposed for three growing seasons to ambient (A = 385 mu mol mol(-1)) and elevated (E = 700 mu mol mol(-1)) CO2 concentrations. Rubisco content was determined by the SDS-PAGE method, Rubisco activity was assayed spectrophotometrically. The highest content of Rubisco enzyme in F. sylvatica was measured immediately after full leaf development followed by a gradual decrease throughout the growing season. By contrast, Rubisco content in P. abies increased markedly during the growing season. In both tree species, down-regulation of Rubisco content in trees cultivated under elevated CO2 concentration was observed. Rubisco activity was stimulated in F. sylvatica by E treatment but not in P. abies. Because no significant differences were found in Rubisco activation state between A and E, we assume that stimulation of Rubisco activity in E is not a consequence of higher carbamylation but could be caused by the release of inhibitors from active sites of Rubisco under elevated CO2. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2013
Počet záznamů: 1