Počet záznamů: 1

Recombinant fragment of an antibody tailored for direct radioiodination

SYSNO ASEP	0378891
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Recombinant fragment of an antibody tailored for direct radioiodination
Tvůrce(i)	Sedláček, Juraj (UMG-J)_RID Fábry, Milan (UMG-J)_RID Sieglová, Irena (UMG-J) Král, Vlastimil (UMG-J)_RID Uhnáková, Bronislava (UMG-J) Mudra, M. (SK) Kronrád, L. (CZ) Sawicka, A. (PL) Mikolajczak, R. (PL) Řezáčová, Pavlína (UMG-J)_RID
Zdroj.dok.	Journal of Labelled Compounds and Radiopharmaceuticals. - : Wiley - ISSN 0362-4803 Roč. 55, č. 1 (2012), s. 52-56
Poč.str.	5 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	I125 labelling ; single-chain antibody variable fragment ; tyrosine-rich polypeptide segment ; fusion protein
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	2A-2TP1/076 GA MPO - Ministerstvo průmyslu a obchodu
	1M0505 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000299555700010
DOI	10.1002/jlcr.1945
Anotace	High specific radioactivity (per mass or per mole) is an important and desirable parameter for the radiopharmaceuticals that are intended to target disease biomarker molecules. In direct radioiodination of immunoglobulins or their fragments, the predominant chemical reaction is usually the monoiodination of the phenol rings of tyrosine amino acid residues. For such radioiodination, the maximum achievable level of labeling of a given protein molecule obviously correlates to the number of solvent-accessible tyrosine residues. The grafting of proteins with additional iodine-acceptor tyrosine residues by conventional chemical techniques has been recently reported. We show here that a recombinant single chain variable fragment (scFv) of an anti-b-III-tubulin antibody, having a potential use in imaging neural lesions or astrocytoma and certain nonneuronal tumors, can be modified in a similar manner. However, in contrast to previously published examples, tyrosine residues are added to the polypeptide main chain without any chemical reaction, solely with molecular cloning and recombinant expression. Importantly, the tailoring outlined here is carried out ‘once and for all’ at laboratory scale (modifying permanently the expressed nucleic acid), whereas chemical indirect methods are necessarily carried out either at production scale or always before each round of radioiodination.
Pracoviště	Ústav molekulární genetiky
Kontakt	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Rok sběru	2013

Počet záznamů: 1