Počet záznamů: 1
The strength of integrin binding between neutrophils and endothelial cells
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SYSNO ASEP 0375893 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The strength of integrin binding between neutrophils and endothelial cells Tvůrce(i) Labrador, V. (FR)
Říha, Pavel (UH-J) SAI, ORCID, RID
Muller, S. (FR)
Dumas, D. (FR)
Wang, X. (FR)
Stoltz, J. F. (FR)Celkový počet autorů 6 Zdroj.dok. European Biophysics Journal With Biophysics Letters - ISSN 0175-7571
Roč. 32, č. 8 (2003), s. 684-688Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova endothelium ; integrins ; neutrophil adhesion ; scanning microscopy Vědní obor RIV BO - Biofyzika CEP GA305/01/1605 GA ČR - Grantová agentura ČR CEZ AV0Z2060917 - UH-J UT WOS 000187309600004 DOI 10.1007/s00249-003-0329-4 Anotace The firm adhesion of activated polymorphonuclear neutrophils to endothelial cells in blood vessels is achieved through binding of the integrin intercellular adhesion molecule. To contribute to the better understanding of this adhesion step, our investigation is aimed at the relationship between integrin expression and the strength of neutrophil binding to endothelial cells. Flow cytometry and 3D scanning microscopy are used to study integrin expression and distribution, respectively. It is found that CD11b/CD18 integrin expression is localized in clusters distributed irregularly over the neutrophil surface. After cell activation, the cluster distribution polarizes, increasing the local CD11b/CD18 density concurrently with nearly doubled integrin expression. The neutrophil adhesion efficiency is measured in a flow chamber coated successively by various substrates, including endothelial cells in an activated state. Analysis of the flow dependence of the number of attached cells reveals the prevailing number of neutrophils with stronger binding to the endothelium when both cells are in the activated state in comparison with non-activated cells. Pracoviště Ústav pro hydrodynamiku Kontakt Soňa Hnilicová, hnilicova@ih.cas.cz, Tel.: 233 109 003 Rok sběru 2013
Počet záznamů: 1